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In protein assay

Each protein in a sample is unique and can demonstrate that individuality in protein assays as variation in the color response. Such protein-to-protein variation refers to differences in the amount of color (absorbance) that are obtained when the same mass (microgram or milligram) of various proteins are assayed concurrently (i.e., in the same run) by the same method. These differences in color response relate to differences among proteins due to amino acid sequence, isoelectric point (pi), secondary structure, and the presence of certain side chains or prosthetic groups. [Pg.98]

Research on microchip protein analysis has been very active for cellular protein functional assay, clinical diagnostics, and proteomics studies. Once again, the microfluidic technology plays an important role in protein assays. Immunoassay, protein separation, and enzymatic assay will be described in detail in subsequent sections. [Pg.337]

Colloidal gold or silver marking can also be used on a microscale in protein assays by electron scanning microcopy. [Pg.96]

Major Applications Display device, pH sensors, inks," photoreceptors,5 lithographic plates, photographic materials, determination of surfactants, - Inbricants, food shelf Ufe,ii-i2 in protein assays, 3 vaginal infection test, detecting proteinsi - ... [Pg.40]

Stutzenberger, F J (1992) Interference of the detergent Tween 80 in protein assays. Anal. Biochem. 207(2) 249-254... [Pg.685]

Finally, some amphiphilic sweeteners, eg, aspartame, saccharin, and neohesperidin dihydrochalcone, have been shown to be capable of stimulating a purified G-protein direcdy in an in vitro assay (136). This suggests some sweeteners may be able to cross the plasma membrane and stimulate the G-protein without first binding to a receptor. This type of action could explain the relatively longer response times and the lingering of taste associated with many high potency sweeteners. [Pg.285]

Recently, a reagent that reacts more efficiently with Cn than Folin-Ciocalteau reagent has been developed for protein assays. Bieinehoninie aeid (BCA) forms a purple complex with Cu in alkaline solution. [Pg.129]

Finally, receptor stimulus can be measured through membrane assays directly monitoring G-protein activation (group IV assays). In these assays, radiolabeled GTP (in a stable form for example, GTPj/S) is present in the medium. As receptor activation takes place, the GDP previously bound to the inactive state of the G-protein is released and the radiolabeled GTP/S binds to the G-protein. This is quantified to yield a measure of the rate of GDP /GTP j/S exchange and hence receptor stimulus. [Pg.84]

Inhibitor assay A suitable amount of inhibitor was preincubated with 0.2 ml of polygalacturonase and buffer in a total volume of 1 ml for 10 minutes at 37°C. Control without inhibitor was run simultaneously. The enzyme reaction was initiated by the addition of 1 ml of substrate solution (1% polygalacturonic acid). The decrease in PG activity was a measure of the inhibitory activity. Proper controls containing only Dieffenbachia extract and no fungal PG in the assay mixture were also run to account for the inherent PG activity, if any, of Dieffenbachia extract. One unit of inhibitor activity is defined as the amount of inhibitor that reduces the polygalacturonase activity under the assay conditions by one unit. Specific activity of the inhibitor is expressed as units per mg protein. [Pg.800]

Extracellular PG activity was not detected in cultures on glucose, a similar situation to that of Fusarium moniliforme (18). In contrast, all our data confirm the presence of the protein and the mRNA of PG in non-inducing conditions our assays did reveal no differences between FORL PG growing on both conditions, regarding migration or other detectable characteristics that could justify the presence of the enzyme and its lack activity. It is posible that a very low concentration of the enzyme results in undetectable activity in enzyme assays. [Pg.890]


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See also in sourсe #XX -- [ Pg.45 ]

See also in sourсe #XX -- [ Pg.45 ]




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Proteins assay

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