Immunoglobulin immobilization

The isoterms of adsorption of maleimide-activated immunoglobulins on the surface of Fe304/DMSA were investigated. The adsorption capacity value was A=92.12mg/g (Table 10.7), with the specific surface area specific = 130 m /g. The release of immunoglobulin, immobilized by means of sulfo-SMCC spacer, in the model medium was 8—12% for 24 h. 

Desorption kinetics for Ig immobilized on the surface of the nanocomposites was studied with the use of physiological solution as a model medium. It has been shown that the release of immunoglobulin immobilized at the surface of Fe304/ PAA and Fe304/ -APS nanocomposites via covalent binding is slow and to a lesser extent than in the case of nonspecific immobilization. The antibodies immobilized at the surface of Fe304/HA and Fe304/HA/Ag nanocomposites showed weak desorption in a model medium. 

Cellulose can be activated by CDI and coupled with the amino groups of peptides or immunoglobulins in aqueous alkaline solution to give immobilized peptides or antibodies such as the immunoglobulin IgG [210] (see also Section 6.2) ... 

Denizli, A., Rad, A.Y., and Piskin, E. (1995) Protein A immobilized polyhydroxyethylmethacrylate beads for affinity sorption of human immunoglobulin G./. Chromatogr. B. Biomed. Appl. 668(1), 13-19. June 9. 

L. Korecka, Z. Bilkova, M. Holeapek, J. Kralovsky, M. Benes, J. Lenfeld, N. Mine, R. Cecal, J.-L. Viovy, and M. Przybylski, Utilization of newly developed immobilized enzyme reactors for preparation and study of immunoglobulin G fragments. Journal of Chromatography, B Analytical Technologies in the Biomedical and Life Sciences 808, 15-24 (2004). 

H. Wang, J.S. Li, Y.J. Ding, C.X. Lei, G.L. Shen, and R.Q. Yu, Novel immunoassay for Toxoplasma gondii-specific immunoglobulin G using a silica nanoparticle-based biomolecular immobilization method. Anal. Chim. Acta 501, 37-43 (2004). 

A very interesting approach was presented recently by Niemeyer et al. [113]. They prepared covalent DNA-streptavidin conjugates to which biotinylated alkaline phosphatase, beta-galactosidase, and horseradish peroxidase, as well as biotinylated anti-mouse and anti-rabbit immunoglobulins, were coupled. Immobilization of DNA-streptavidin conjugates was performed by hybridization with the complementary oligonucleotides, bound to the surface. It was demonstrated... 

Selected entries from Methods in Enzymology [vol, page(s)j Detection and quantification, 74, 608-616 effect of antigen/anti-body ratio, 74, 613, 614 applications, 74, 614-615 controls, 74, 612 data analysis, 74, 612-613 immunoglobulin G [conjugation to glucose oxidase, 74, 610 heat aggregation, 74, 609-610 heat denaturation, 74, 610 immobilization, 74, 610] precision, 74,... 

Since the immunoglobulins purified by this method are mostly used for immobilization, buffers containing NH2 groups, as Tris or glycine, should be avoided. 

Table 4.8. Binding affinities of immunoglobulin subclasses from different species to immobilized Protein A, Protein G, Protein L, and thiophilic chromatography supports...

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