Immunoglobulin G IgG

FIGURE 1.11 Three -dimensional spacefilling representation of part of a protein molecule, the antigen-binding domain of immunoglobulin G (IgG). Immunoglobulin G is a major type of circulating antibody. Each of the spheres represents an atom in the structure. 

Secondary antibody and determination. A secondary antibody labeled with an enzyme is added which binds to the primary antibody that is bound to the coating antigen. If the primary antibody were produced in a rabbit, an appropriate secondary antibody would be goat anti-rabbit immunoglobulin G (IgG) conjugated with horseradish peroxidase (HRP) (or another enzyme label). Excess secondary antibody is washed away. An appropriate substrate solution is added that will produce a colored or fluorescent product after enzymatic conversion. The amount of enzyme product formed is directly proportional to the amount of first antibody bound to the coating antigen on the plate and is inversely proportional to the amount of analyte in the standards. 

Figure 2 shows the most abundant class of antibodies found in blood serum and lymph - immunoglobulin G (IgG). IgG of molecular mass about 156 000, is most frequently used as a receptor in immunosensors. According to X-ray data6 8, IgG is a Y-shaped molecule consisting of two identical antigen binding Fab arms of dimensions 6.5 nm by 3.5 nm and an inactive Fc shank of dimensions 5 nm by 3.5 nm. 

The answer is a. (Katzung, p 162.) Many drugs can cause an immunohemolytic anemia. Methyldopa may cause a positive Coombs test in as many as 20% of patients, along with hemolytic anemia. Other drugs with similar actions on red blood cells are penicillins, quinidine, procainamide, and sulfonamides. These form a stable or unstable hapten on the red cell surface, which induces an immune reaction I immunoglobulin G (IgG) antibodies] and leads to dissolution of the membrane. 

Human health effects, of polychlorinated biphenyls, 13 140-142 Human IgG (hlgG), detection of, 14 155, 156. See also Immunoglobulin G (IgG) Human immunodeficiency virus (HIV). See also HIV entries Nevirapine entries inactivation of, 12 139 lactoferricins and, 18 258... 

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