Immunoglobuline G

This experiment describes the adaptation of the bicinchoninic acid (BCA) protein assay to a flow injection analysis. The assay is based on the reduction of Cu + to Cu+ by the protein, followed by the reaction of Cu+ with bicinchoninic acid to form a purple complex that absorbs at 562 nm. Directions are provided for the analysis of bovine serum albumin and rabbit immunoglobulin G, and suggestions are provided for additional analyses. 

Type I allergies are mediated by immunoglobulin E (IgE). Unlike the other immunoglobulins (G,M,A, and D), which are part of the essential defense... 

FIGURE 7.17 Separation of a complex mixture on Fractogel EMD BioSEC (S) with a column dimension of 1000 X 50 mm (Superformance glass column). The sample contained ferritin (I), immunoglobulin G (2), transferrin (3), ovalbumin (4), myoglobin (5), aprotinin (6), and vitamin B, (7). Five milliliters of the mixture was injected onto the column at a flow rate of 3 ml/min (eluent 20 mAI sodium phosphate buffer, 0.1 M NaCI, pH 7.2). 

FIGURE 1.11 Three -dimensional spacefilling representation of part of a protein molecule, the antigen-binding domain of immunoglobulin G (IgG). Immunoglobulin G is a major type of circulating antibody. Each of the spheres represents an atom in the structure. 

T. K. Nadler, S. K. Paliwal and F. E. Regnier, Rapid, automated, two-dimensional liigh-performance liquid cliromatographic analysis of immunoglobulin G and its multimers , 7. Chromatogr. A 676 331-335 (1994). 

A Type III allergic reaction occurs when antibodies of the immunoglobulin G class (IgG) form immune complexes which are slowly eliminated and thus may elicit an inflammatory reaction by binding to the Fey receptors of leukocytes resulting in their activation. 

CN immunoglobulin G (human-mouse monoclonal c7E3 clone p7E3VHhCy4 Fab fragment antihuman... 

TsujimuraY, ObataK, Mukai K, Shindou H, Yoshida M, Nishikado H, Kawano Y, Minegishi Y, Shimizu X Karasuyama H Basophils play a pivotal role in SS immunoglobulin-G-mediated but not immuno-globulin-E-mediated systemic anaphylaxis. Immunity 2008 28 581-589. 

Immunoglobulins A, E, G, M IgG I Immunc obulin G class 1 IgGa, Immunoglobulin G class 2a IGF-I Insulin-like growth factor Ig-SF Immunoglobulin supergene family... 

PAI Plasminogen activator inhibitor PA-IgG Platelet associated immunoglobulin G PAM Pulmonary alveolar macrophages... 

Secondary antibody and determination. A secondary antibody labeled with an enzyme is added which binds to the primary antibody that is bound to the coating antigen. If the primary antibody were produced in a rabbit, an appropriate secondary antibody would be goat anti-rabbit immunoglobulin G (IgG) conjugated with horseradish peroxidase (HRP) (or another enzyme label). Excess secondary antibody is washed away. An appropriate substrate solution is added that will produce a colored or fluorescent product after enzymatic conversion. The amount of enzyme product formed is directly proportional to the amount of first antibody bound to the coating antigen on the plate and is inversely proportional to the amount of analyte in the standards. 

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