Immunoelectrophoretic methods

Much of the early work on the development of the proteins in tropical populations, utilizing mainly electrophoretic technique, have been summarized (E6). Using both the quantitative electrophoretic and qualitative immunoelectrophoretic methods to study the developmental patterns of... 

Lowenstein H, Aukrust L, Gravesen S Cladosporium herbamm extract characterized by means of quantitative immunoelectrophoretic methods with special attention to immediate type allergy. Int Arch Allergy Appl Immunol 1977 55 1-12. 

Tanahashi et al. (1968) compared the immunological properties of bovine, water buffalo, ovine, caprine, porcine, guinea pig, and human a-lactalbumins by the method of Oudin. They found that the nonruminant a-lactalbumins do not react with antisera to the bovine protein. This is in accordance with our experience (K. Bell and H. A. McKenzie) using the Ouchterlony and immunoelectrophoretic methods. However, Sakar et al. (1971) found that while bovine, water buffalo, and caprine a-lactalbumins exhibit extensive cross-reaction in the Ouchterlony test, these ruminant a-lactalbumins may be differentiated quanti-... 

The most often used procedures for evaluating the specificity of antibody preparations are double diffusion methods developed by Ouchterlony and immunoelectrophoretic methods developed by Grabor and Williams. For a discussion of variations of these techniques as well as other useful procedures the reader is referred elsewhere (7-9). 

Sensidvity of the immunoelectrophoretic methods may be increased by using radioacdve (5) or enzyme (6) labeling of andgen or antibody. 

Antigen-antibody binding interactions can be induced after an electrophoretic separation pattern is blotted from the gel onto a membrane, in a technique called immunoblotting, and will also occur directly in the gels used for electrophoresis. Immunoelectrophoretic methods are used for the identification and quantitation... 

V3. Verbruggen, R., Quantitative immunoelectrophoretic methods A literature survey. Clin. Chem. 21, 5-43 (1975). 

The ease with which individual proteins may be assessed and quantified using immunoelectrophoretic methods has resulted in widespread use of these methods when there is a requirement for specific assessment of a protein. This has been seen especially in clinical chemistry, where numerous measurements of... 

Several immunochromatographic and immunoelectrophoretic methods that have been used for small (hapten) molecules, to the authors knowledge, have not been... 

Most sample components analyzed with electrophoretic techniques are invisible to the naked eye. Thus methods have been developed to visualize and quantify separated compounds. These techniques most commonly involve chemically fixing and then staining the compounds in the gel. Other detection techniques can sometimes yield more information, such as detection using antibodies to specific compounds, which gives positive identification of a sample component either by immunoelectrophoretic or blotting techniques, or enhanced detection by combining two different electrophoresis methods in two-dimensional electrophoretic techniques. 

Kerenyi L, Galiyas F (1972) A highly sensitive method for demonstrating proteins in electrophoretic, immunoelectrophoretic and immunodiffusion preparations. Clin Chim Acta 38 465 167... 

Two major variations of the method are used at present. The first approach is closely related to some immunoelectrophoretic techniques, e.g., crossed Immunoelectrophoresis or rocket electrophoresis [180,181], the only difference is that usually a lectin instead of an antibody is incorporated in the agarose gel the conditions in the gel (electroendoosmosis and pH) are used such that the lectin has a very low electrophoretic mobility. The lectin reacts with specific glycoproteins and forms characteristic precipitation lines analogously to the reactions observed during Immunoelectrophoresis between the antibody and the antigen. This method can be... 

Electrophoretic methods use various support media, buffers, voltage, amperage, and times. The media include agarose gel, cellulose acetate, starch gel, and polyacrylamide gel. Techniques include single dimensional electrophoresis, crossed immunoelectrophoresis, and immunoelectrophoretic fixation (lEF). 

Gel procedures originate from the classical immunoelectrophoresis of P. Grabar, often referred to as lEA, an abbreviation for immunoelectrophoretic analysis, and from the crossing diagrams of K. Nakamura. A distinction may be made between the two-dimensional QE of Clarke and Freeman (Figure 1) and the one-dimensional rocket immunoelectrophoresis of Laurell, often referred to as electroimmunoassay (EIA). Numerous technical modifications of these methods have been devised in order to obtain a certain characterization of one or more proteins. The methods are suitable for establishing an inventory of proteins and for molecular characterization of... 

Immunoelectrophoretic Analysis. The immunoelectrophoretic studies were performed according to the method of Scheidegger (1955). The rabbit anti-HMG serum was prepared by immunizing the animals with roughly purified HMG. Rabbit anti-human albumin serum... 

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