Immunocytochemical techniques for

Approaches that have been used to prove that a suspected transmitter resides in the presynaptic terminal of the pathway under study include biochemical analysis of regional concentrations of suspected transmitters and immunocytochemical techniques for enzymes and peptides. 

Beaudet A, Doumaud P, Boudin H. Complementarity of radioautographic and immunocytochemical techniques for localizing neuroreceptors at the light and electron microscopic level. Braz J Med Biol Res 1998 31 215-223. 

Eckert, M, and Ude, J. (1983) Immunocytochemical techniques for demonstration of peptidergic neurons, mFunctional Neuroanatomy (Strausfeld, N. J, ed.), Spnnger, Berlin, pp. 267—301... 

Eckert M, Ude J (1983) Immunocytochemical techniques for the identification of peptidergic neurons. Functional neuroanatomy. Springer, Berlin, pp 268-301... 

Several additional strategies have been employed as aids in the diagnosis of PDC deficiency. These include (1) mutational analysis, based on sequencing of genomic DNA, complementary DNA, or both (2) visualization of the proteins of the complex by electrophoresis/ immunoprecipitation or electrophoresis/im-munoblotting techniques and (3) most recently, an immunocytochemical method for assaying the content of El a protein in individual patient cells. 

Palacios, G., A double immunocytochemical and histochemical technique for demonstration of cholinergic neurons and microglial cells in basal forebrain and neostriatum of the rat, Neurosci. Lett., 115, 13, 1990. 

Immunocytochemical labeling methods and related techniques for ultrastmctural analysis of neuronal connectivity. In Merighi A, Carmignoto G (eds) CeUular and molecular methods in neuroscience research. Springer, New York, pp 161-180... 

The application of immunocytochemical techniques to the study of nervous tissue and organs had initially to face a series of technical problems that made it a very demanding procedure for obtaining successfiil immunostaining in combination with adequate structural preservation, particularly at the electron microscope level. 

Montone KT, Brigati DJ, Budgeon LR. Anatomic viral detection is automated the application of a robotic molecular pathology system for the detection of DNA viruses in anatomic pathology substrates, using immunocytochemical and nucleic acid hybridization techniques. Yale J. Biol. Med. 1989 62 141-158. 

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