Anti-actin antibody

SMA Smooth muscle antibodies were first described by G.D. Johnson et al. (1965). They mostly possess anti-actin (generally F-actin) specificity, predominantly of the IgG type. Antibodies against troponin and a-actin may also be present. There is great heterogeneity, and a mixture of different antibodies can almost always be found. Evidence of anti-actin antibodies has a high diagnostic value, particularly with a titre of > 1 640 and in the presence of an IgG type. (s. fig. 5.12) (s. p. 119)... 

Alternatively the cells are metabalically labeled with 50(j.Ci [2- H]-adenine per ml of medium for 16 h (Staddon etal., 1991). Labeled actin can be identified by immunoprecipitation with anti-actin antibody (from Boehringer or ICN) or by immunoblot analysis of cell lysates electrophoretically resolved on 2D gels. 

First, an overlay assay was used to demonstrate binding of photoaffinity labeled FPR to actin from neutrophil cytosol [49]. Second, actin increased the sedimentation rate of Triton X-lOO-solubilized FPR in a sucrose density gradient [49]. Third, anti-actin antibodies were able to immunosediment FPR suggesting... 

Primary antibody Monoclonal anti-GAPDH antibody and monoclonal anti-p-actin antibody from mouse (see Note 1). 

Fig. 13.1 Cytoprotective action of adenosine in a quantitative model of mouse hindlimb ischemia and reperfusion (I/R) injury. Adult wild type mice were injected with (a) sterile vehicle (0.1% DMSO in phosphate-buffered saline, pH 7.4) or various adenosine receptor agonists (b, c) or antagonist (d, f). (a) Following ischemia and reperfusion, skeletal muscle showed a significant uptake of Evans Blue dye (EBD) in vehicle-treated mice (first part of (a), representative of 7 mice). The contra-lateral leg not subjected to ischemia-reperfusion showed virtually no EBD uptake. In the second part of (a), the same section was stained with rabbit polyclonal anti-skeletal muscle actin antibodies followed by staining with goat anti-rabbit IgG conjugated with FITC. (b) The nonselective adenosine agonist R-PIA caused a large reduction in the EBD-stained area (see both first and second parts to this figure, representative of six mice)...

Figure 2. Cultured pulmonary artery endothelial cells stained for tubulin (red), actin (green) and DNA (blue). The dual immunofluorescence procedure used rabbit anti-actin IgG and mouse anti-alpha tubulin IgG as primary antibodies. The secondary antibodies used were Texas Red-conjugated goat, anti-rabbit IgG and FITC-conjugated goat, anti-mouse IgG. The sample was also stained with the DNA-specific dye Hoechst 33342. Scale bar is equal to 20 microns.

Islam S, Mekhloufi F, Paul JM, Islam M, Johanet C, Legendre C, Degott C, Abuaf N, Homberg JC. Characteristics of clometacin-induced hepatitis with special reference to the presence of anti-actin cable antibodies. Autoimmunity 1989 2(3) 213-21. 

Gugliotta P, Sapino A, Macri L, et al. Specific demonstration of myoepithelial cells by anti-alpha smooth muscle actin antibody. J Histochem Cytochem. 1988 36 659-663. 

Colitis was induced by 2% DSS in the drinking water. Methylglyoxal-modified proteins were identified by immunoblot analysis using anti-argpyrimidine monoclonal antibody (A). Hsp25 and /3-actin were identified by immunoblot analysis using anti- Hsp25 antibody (B) and anti- fi-actin antibody (C), respectively. 

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