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Immunoassay rabbit immunoglobulin

H.C.B. Graves, The effect of surface-charge on non-specific binding of rabbit immunoglobulin-G in solid-phase immunoassays. J. Immunol. Methods 111, 157—166 (1988). [Pg.400]

Siddle, K., and Soons, M., Monoclonal antibodies for human pituitary glycoprotein hormones and rabbit immunoglobulin. In "Monoclonal Antibodies and Developments in Immunoassay" (A. Albertini and R. Ekins, eds.), pp. 53-66. Elsevier, Amsterdam, 1981. [Pg.108]

Fig. 9.10. Matrix eflFects in immunoassay are generally revealed as a decrease in absolute signal at any level of spiked anal5d e concentration. This effect decreases with the dilution of the sample. Example presented an indirect competitive ELISA for 4-nitrophenol in milk at different dilutions. A 4-nitrophenol derivative coupled to bovine serum albumin was used as the competitor, an anti-4-nitrophenol (polyclonal, from rabbit) as the antibody and goat anti-rabbit immunoglobulin G coupled to peroxidase as the detection system [43]. (Reprinted with permission.)... Fig. 9.10. Matrix eflFects in immunoassay are generally revealed as a decrease in absolute signal at any level of spiked anal5d e concentration. This effect decreases with the dilution of the sample. Example presented an indirect competitive ELISA for 4-nitrophenol in milk at different dilutions. A 4-nitrophenol derivative coupled to bovine serum albumin was used as the competitor, an anti-4-nitrophenol (polyclonal, from rabbit) as the antibody and goat anti-rabbit immunoglobulin G coupled to peroxidase as the detection system [43]. (Reprinted with permission.)...
In the first reported example of chemiluminescence energy transfer, the iso-luminol derivative ABEI (Fig. 14) was covalently attached via its isothiocyanate to either rabbit immunoglobulin or cyclic AMP. Antibodies to these molecules were labeled with fluorescein. Homogeneous competitive immunoassays were conducted for both analytes and, in order to maximize the combined effects of fluorescein emission and isoluminol quenching, the parameter measured, as a function of analyte concentration, was the intensity ratio at the wave-... [Pg.118]

A 3-maleimidobenzoyl derivative of L-thyroxine methyl ester has also been coupled to jS-D-galactosidase and used in an enzyme immunoassay of L-thyroxine. The procedures for the conjugation of rabbit immunoglobulin G and Fab antibodies with 3-D-galactosidase using AA -2-phenylene-dimaleimide have been improved by altering the reaction conditions. ... [Pg.673]

Rabbit immunoglobulin IgG anti-(human immunoglobulin IgG) antibody Enzyme-linked immunoassay of human immunoglobulin IgG 63... [Pg.436]

Rabbit anti-(human immunoglobulin IgG) antibodies, horse anti-(rabbit immunoglobulin) antibodies, and ovalbumin, all labelled with fluorescein isothiocyanate, and goat anti-(rabbit immunoglobulin) antibodies labelled with tetramethylrhodamine isothiocyanate have been used with insolubilized antigens in new microfluorometric immunoassays. ... [Pg.472]

Reduced rabbit immunoglobulin IgG coupled to jS-o-galactosidase has been used in an enzyme-linked immunoassay for human immunoglobulin IgG. ... [Pg.472]

P-D-Galactosidases.—jS-o-Galactosidase derivatized with either reduced rabbit immunoglobulin IgG or progesterone has been used in enzyme-linked immunoassays of human immunoglobulin IgG and progesterone, respectively. [Pg.478]

Purification of rabbit immunoglobulin Ig anti-(porcine pancreatic a-amylase) antibody solid-state immunoassay of a-amylase... [Pg.484]

Liburdy, R. P. Rabbit immunoglobulin-N-(3-pyrene)-maleimide conjugate for fluorescent immunoassay. U.S. Patent 4207075,1980. [Pg.358]


See other pages where Immunoassay rabbit immunoglobulin is mentioned: [Pg.269]    [Pg.473]    [Pg.252]    [Pg.361]    [Pg.246]    [Pg.246]    [Pg.450]    [Pg.256]    [Pg.257]    [Pg.366]    [Pg.148]    [Pg.273]    [Pg.385]    [Pg.48]    [Pg.181]    [Pg.196]    [Pg.264]    [Pg.758]    [Pg.250]    [Pg.475]   
See also in sourсe #XX -- [ Pg.361 , Pg.364 ]




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