Energy transfer immunoassay

Hildebrandt N, Charbonniere LJ, Lohmannsroben HG (2007) Time-resolved analysis of a highly sensitive forster resonance energy transfer immunoassay using terbium complexes as donors and quantum dots as acceptors. J Biomed Biotechnol 2007 79169... 

Charbonniere LJ, Hildebrandt N, Ziessel RF, Lohmannsroben HG (2006) Lanthanides to quantum dots resonance energy transfer in time-resolved fluoro-immunoassays and luminescence microscopy. J Am Chem Soc 128 12800-12809... 

Sq635-b and Sq660 were also utilized as donor-acceptor pairs in combination with an HSA/anti-HSA system, in a fluorescence energy transfer (FRET)-based immunoassay [95, 96]. 

Oswald B, Gruber M, Bohmer M, Lehmann F, Probst M, Wolfbeis OS (2001) Novel diode laser-compatible fluorophores and their application to single molecule detection, protein labeling and fluorescence resonance energy transfer immunoassay. Photochem Photobiol 74 237-245... 

Schobel, U., Egelhaaf, H. J., Brecht, A., Oelkrug, D. and Gauglitz, G. (1999). New donor-acceptor pair for fluorescent immunoassays by energy transfer. Bioconjug. Chem. 15, 1107-14. 

Khanna, P.L., and Ullman, E.F. (1980) 4, 5 -dimethoxy-6-carboxyfluorescein A novel dipole-dipole coupled fluorescence energy transfer acceptor useful for fluorescence immunoassays. Anal. Biochem. 108,156. 

Like FRET, today BRET is predominantly used in biological sciences, especially in the monitoring of protein-protein interactions such as hormone-receptor interaction [223, 224] and protein-DNA interaction in living systems. However, BL resonance energy transfer can also be applied in immunoassays by using for instance a peptide-tagged luciferase and a fluorescein-labeled antipeptide antibody [225]. The development of more BRET assays for small-molecule analytes is thus awaited. 

Yamakawa Y, Ueda H, Kitayama A et al (2002) Rapid homogeneous immunoassay of peptides based on bioluminescence resonance energy transfer from firefly luciferase. J Biosci Bioeng 93 537-542... 

Morrison LE (1988) Time-resolved detection of energy transfer theory and application to immunoassays. Anal Biochem 174 101-20... 

J. R. Lakowicz and B. P. Maliwal, Fluorescence lifetime energy transfer immunoassay quantified by phase-modulation fluorometry, Sensors andActuators B 12, 65-70 (1993). 

An immunosensor based on a competitive fluorescence energy-transfer immunoassay was reported by Anderson 105) for the measurement of phenytoin. Texas red-labeled antibody was incubated with a phenytoin derivative. On displacement of the derivative by the antigen, the change in the fluorescence signal was recorded. Detection limits approached 5 /iM with response times ranging from 5 to 30 min. 

P. Khanna, Energy transfer immunoassays using phycobiliproteins. Presentation at Conference of Phycobiliprotein in Biology and Medicine, Seattle, Washington, September 9-10, 1985. 

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