Immunoassay digoxin

EN107 Kildal-Brandt, P., Weber, T. and Sutherland, J.W. (1992). Performance on three thin-film enzyme immunoassays Digoxin, phenytoin and phenobarbital using two rate algorithms. Clin. Chem. 38, 1093, Abstr. 681. 

Bioluminescence can also be used as the basis for immunoassay. For example, bacterial luciferase has been used in a co-immobilized system to detect and quantify progesterone using a competitive immunoassay format (34), and other luciferase-based immunoassays have been used to quantify insulin, digoxin, biotin, and other clinically important analytes (35). 

A competitive electrochemical enzyme immunoassay has been demonstrated for digoxin Alkaline phosphatase, which catalyzes the hydrolysis of phenyl phosphate... 

Homogeneous electrochemical enzyme immunoassays for both phenytoin and digoxin have been developed. In both cases the label was glucose-6-phosphate dehydrogenase, which catalyzes the reduction of NAD to NADH. The NADH produced was detected by LCEC at a carbon paste electrode. 

Digoxin-like immunore active substances (found in patients with chronic heart failure, end stage renal disease, liver disease, or third trimester of pregnancy) may cross-react with certain digoxin immunoassays and may result in a false elevation of levels... 

K.R. Wenmeyer, H.B. Halsall, W.R. Heineman, C.P. Voile, and I.W. Chen, Competitive heterogeneous enzyme immunoassay for digoxin with electrochemical detection. Anal. Chem. 58, 135-139 (1986). 

M.Y. Keating and G.A. Rechnitz, Potentiometric enzyme immunoassay for digoxin using polystyrene beads. Anal. Lett. 18, 1-10 (1985). 

H. Qi and C. Zhang, Homogeneous electrogenerated chemiluminescence immunoassay for the determination of digoxin. Anal. Chim. Acta 501, 31-35 (2004). 

P. Helsingius, 1. Hemmila, and T. Lovgren, Solid-phase immunoassay of digoxin by measuring time-resolved fluorescence, Clin. Chem. 32, 1767-1769 (1986). 

M. Plebani and A. Burlina, Fluorescence energy transfer immunoassay of digoxin in serum, Clin. Chem. 31, 1879-1881 (1985). 

Chow L, Johnson M, Wells A, Dasgupta A. Effect of the traditional Chinese medicines Chan Su, Lu-Shen-Wan, Dan Shen, and Asian Ginseng on serum digoxin measurement by Tina-quant (Roche) and Synchron LX system (Beckman) digoxin immunoassays. J Clin Lab Anal 2003 17 22-27. 

These PVC based ionophore carrying matrices are also membranes which can be adapted for immunoassay systems. Keating and Rechnitz (40) have described an antigen digoxin coupled to one of the potassium ionophores (eg cw-dibenzo-18 crown-6) and the resulting conjugate immobilised in the PVC membrane. 

The automated system of the particle counting immunoassay is now commercially available as the product impact (Immunoassay by Particle Counting), which can measure C-reactive protein, ferritin, human placental lactogen, thyroxine, a-fetoprotein (C7), IgE (M2), digoxin (C6), somatotropin (C5), and others. 

F2. Freytag, J. W., and Litchfield, W. J., Liposome-mediated immunoassays for small haptens (digoxin) independent of complement. J. Immunol. Methods 70, 133-140 (1984). 

Separation-free, homogeneous immunoassay protocols offer several advantages in comparison to heterogeneous methods. Because no separation is involved, the number of procedural steps is decreased, which decreases the time required per assay. Additionally, because the physical transfer step is avoided, potential sample loss related to this step is eliminated. Drugs with low molecular weights (amphetamines, digoxin) are commonly measured by separation-free homogeneous immunoassay protocols. ... 

Fushimi R, Tachi J, Amino N, Miyai K. Chinese medicine interfering with digoxin immunoassays. Lancet 1989 1(8633) 339. 

Pleasants RA, Williams DM, Porter RS, Gadsden RH Sr. Reassessment of cross-reactivity of spironolactone metabolites with four digoxin immunoassays. Ther Drug Monit 1989 ll(2) 200-4. 

Capone D, Gentile A, Basile V. Possible interference of digoxin-like immunoreactive substances using the digoxin fluorescence polarization immunoassay. J Appl Ther Res 1999 2 305-8. 

E321 Danielson, S.J., Detty, M.R. and Alexandrovich, S.K. (1987). Improved labels for use in digoxin multilayer enzyme immunoassays. Clin. Chem. 33,923, Abstr. 211. 

EN90 Danielson, S.J., Ponticello, N., Sutton, R., Oenick, M. and Swartz, J. (1992). Novel carboxylic acid copolymer beads and thdr use in thin-film immunoassays for thyroxine, phenytoin, phenobarbital and digoxin. Clin. Chem. 38, 1096, Abstr. 696. 

Bahar, I., Vaupel, U., Case, A., Farrell, K., Inbar, S., and Metzmann, E. (1990). Rapid self- contained immunoassay for digoxin. Clin. Chem. 36, 1028, Abstr. 359. 

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