Immunoassay liposome-mediated

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Liu, X Huan, S. Bu, Y Shen, G. Yu, R. Liposome-mediated enhancement of the sensitivity in immunoassay based on surface-enhanced Raman scattering at gold nanosphere array substrate. Talanta 2008, 75, 797-803. 

Immunoassays have been based on the potentiometric measurement of marker ions such tetrapentylammonium ion (TPA ) that are loaded in phospholipid liposomes Complement mediated immunolysis of these loaded vesicles is caused... 

As an alternative to the catalytic activity of a conjugated enzyme, the complement-mediated immunolysis of liposomes can be used to amplify the measuring signals of immunoassays. The liberation of liposome-entrapped markers depends on the amount of antibody adhering to the lipid membrane and serves to measure the concentration of antigen. Such liposomes may be filled with quarternary ammonium ions detectable by ion sensitive electrodes (Umezawa et al., 1983), with enzyme substrates, e.g. glucose, which can be measured with enzyme electrodes... 

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