Immunization immunoglobulin separation

May interfere with the immune response to live virus vaccines Drug interactions have not been evaluated. However, it is recommended that immunoglobulin be administered separately from other agents... 

Null cells are a separate subset of lymphocytes that lack surface markers of B or T origin. These cells, also referred to as large granular lymphocytes, are thought to perform functions such as direct cytotoxicity to foreign entities, and they act either alone (natural killer cells) or in concert with immunoglobulin (antibody-dependent cellular cytotoxicity). (Further details regarding lymphocytes are found in Chap. 84, on the immune system.)... 

About 10% of serum proteins are immunoglobulins (Igs). After immunization, the specific antibodies produced are about 1"C5% of this fraction, so the required Ig (in ELISA) may be from 0.1 to 2.5% of the total protein in a serum. Some assays are favored by the relatively crude fractionation of serum to obtain Igs, e.g., for use in binding to plates in trapping (sandwich assays) to avoid competition for plastic binding sites by other serum proteins. Several methods for separation of Igs are available for use in ELISA. These procedures are suitable for polyclonal antibodies but not necessarily for mAbs. The isolation of total Igs as compared to the purification of specific Igs, is relatively simple. 

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