Immobilized biomimic

It should be noted that the mechanism of catalyst deactivation (7.2) is a particular case, because the immobilized biomimic is protected from this danger. 

In order to obtain a fragment of catalase, it was immobilized by adsorption on various organic and inorganic carriers (such as aluminum oxide, diasorb DEAE, agarose), with further tryp-sine treatment. Aluminum wire or foil was used as the electrode. Such electrodes were selected because of their low price and inertness in relation to hydrogen peroxide. The binding agent between the electrode and biomimic was Pattex adhesive and 7.5% polyacrylamide gel. 

The possibility of having membrane systems also as tools for a better design of chemical transformation is today becoming attractive and realistic. Catalytic membranes and membrane reactors are the subject of significant research efforts at both academic and industrial levels. For biological applications, synthetic membranes provide an ideal support to catalyst immobilization due to their biomimic capacity enzymes are retained in the reaction side, do not pollute the products and can be continuously reused. The catalytic action of enzymes is extremely efficient, selective and highly stereospecific if compared with chemical catalysts moreover, immobilization procedures have been proven to enhance the enzyme stability. In addition, membrane bioreactors are particularly attractive in terms of eco-compatibility, because they do not require additives, are able to operate at moderate temperature and pressure, and reduce the formation of by-products. 

Qingwen, L. et al.. Immobilization of glucose oxidase in sol-gel matrix and its application to fabricate chemiluminescent glucose sensor. Mater. Sci. Eng. C Biomim. Supramol. Syst., 11, 67, 2000. 

Teles ERR, Fonseca LP (2008) Applications of polymers for biomolecule immobilization in electrochemical biosensors. Mater Sci Eng C Biomim Supramol Syst 28 1530-1543... 

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