ID Planar Gel Electrophoresis

Prior to the development of CZE, the primary application of electrophoresis was for large biological macromolecule separations on planar gels. The gels were supported on glass plates not unlike the particles in the thin layer of a TLC plate. These so-called slab gels were needed to confine the separation buffer. It was easier to prepare cross-linked chemical gels on the open surface than in narrow capillaries and to remove the gel from the support plate to prepare a fresh slab gel for a new analysis. Electrophoretic separations of multiple samples in ID could be performed and easily visualized and compared. This was the standard tool for biochemists 

1 Compare packed column LC, capillary column LC, and CZE (as best, intermediate, poorest, or inapplicable for the following categories)  

Describe for the following HPLC procedures. (1) What is meant by these (2) What is the purpose of these (3) How is it done  

4 Describe three ways in which an HPLC support particle differs from one used in packed column GC. 

5 Describe the primary purpose and advantages of using the following columns in HPLC  

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