Hyflo Super Cel

Diatomaceous earth (Celite 535 or 545, Hyflo Super-cel, Dicalite, Kieselguhr) is purified before use by washing with 3M hydrochloric acid, then water, or it is made into a slurry with hot water, filtered at the pump and washed with water at 50° until the filtrate is no longer alkaline to litmus. Organic materials can be removed by repeated extraction at 50° with methanol or chloroform, followed by washing with methanol, filtering and drying at 90-100°. 

The culture broth (about 1,100 gallons in volume) is adjusted to pH 9.5 with 40% sodium hydroxide solution and is filtered to remove the mycelium, the filtration being assisted by use of 3% of Hyflo Super-Cel, a filter aid, (sold by Johns-IVlanville Company). The clear filtrate is extracted with amyl acetate in a Podbielniak extractor using a ratio of 1 volume of amyl acetate to 6 volumes of clarified broth. The amyl acetate extract is in turn extracted batchwise with water brought to about pH 5 by the addition of sulfuric acid. Two... 

Preparation of oi-Acetylamino-cx-Carboxy- -(S-Benzyloxy-lndolyl-3j-Propionic Acid 18 grams ethyl o -acetylamino-0 -carbethoxy-/3-(5-benzyloxy-lndolyl-3)-propionate was suspended in 85 ml water containing 8.5 grams sodium charcoal. The suspension was refluxed for 4 hours, decolorizing charcoal added, and the solution filtered hot through Hyflo Super-cel. 

The low-temperature method was then applied to the resolution of ( )-2-hydroxy-2-(pentafluorophenyl)acetonitrile (7) (Fig. which is usahle for the syntheses of a variety of ethane diols, amino alcohols containing CgFj groups as novel chiral ligands. After screening lipases such as Amano PS and AK, lipase LIP Pseudomonas aeruginosa lipase immobilized on Hyflo Super-Cel, Toyobo,... 

Parathion is a relatively unstable compound and the drastic treatment with sulfuric acid cannot be used. Interfering extractives from some plants can be removed without loss of parathion by passing the chloroform solution through a short column containing 2 parts of Attaclay plus 1 part of Hyflo Super-Cel, followed by rinsing with an equal volume of pure chloroform. DDT also passes through such a column without loss. The solution is then evaporated in a test vial as described before. 

Mix equivalent weights of silica gel 60 GF254 (Merck) and diatomaceous earth (Hyflo Super-Cel Celite, World Minerals) in a mechanical blender or in a large plastic bag for 2 hr. Store <3 months in a sealed container at room temperature. 

Keywords tiiiol, Hyflo Super Cel, microwave irradiation, oxidative coupling, disulfide... 

Reaction mixtures were prepared by mechanical dispersion of the thiol on the support (Hyflo Super Cel, Fulka) with a vortex mixer, or by impregnation of the... 

When Upases are immobiUzed on Celite they can readily be used in dry organic solvents. Pseudomonas cepacia (also named B. cepacia) Upase was immobiUzed in the presence of sucrose on Hyflo super-cel CeUte [36, 40, 41] and used in the first enantioselective synthesis of cyanohydrin acetates via a DKR. Similarly, other successful syntheses of cyanohydrin acetates via DKR were catalyzed by Upases immobiUzed on CeUte [42]. This is in contrast to Novozym 435, which had successfully been used for the DKR of aUphatic cyanohydrin acetates (see Section 2.2.1 and Scheme 2.2) [29]. When Novozym 435 was used for the enantioselective synthesis of mandelonitrile acetate (la) via DKR, the reaction did not proceed [43]. It was... 

A special TLC material, kieselguhr, originated from diatomaceous earth which is thermally treated and granulated to a specific particle size (such as between 5 and 40 pm). Kieselguhr has an extremely low specific surface, 1-5 m /g, therefore it is inert. Similar materials are known under various trade names such as Celit, Filter Cel, Hyflo Super Cel etc. [431. 

The Pseudomonas aeruginosa lipase (immobilised on hyflo Super-Cel) catalysed kinetic resolution of (rac)-2-(acetyloxy)-2-(pentafluorophenyl)acetonitrile gave en-antiomerically pure cyanohydrin and its antipodal ester [142-144l... 

To a suspension of 100 g of baker s yeast in 125 mL of water are added 1.14 g (5 mmol) of octyl 2-mcthvl-3-oxobutanoate and the suspension is stirred at 30 CC. Six 2.5-g portions of glucose arc added at 6-h intervals. After 2 d. ethyl acetate and then Celite (Hyflo Super-Cel) are added to the suspension and the mixture is filtered. The Celite layer is washed three times with ethyl acetate. The combined ethyl acetate layer is washed with water, dried over anhyd Na,S04. and evaporated in vacuo. The synjanti ratio is measured by N.MR spectroscopy. The product is subjected to column chromatography (silica gel, hexane/ethyl acetate 9 1), to give octyl (2/f,3S)-3-hydroxy-2-mcthylbutanoate yield 0.93g (82%) [ijp4 +3.33 (r = 3.00, CHClj) 100% ce. 

Hyflo Super-Cel. [Manville] Diatoma-ceous earth filin aid. 

Sample preparation Condition a 500 mg Bond Elut C18 SPE cartridge with 5 mL MeOH and 10 mL water. Homogenize a 5 g tissue sample with 100 mL MeCN 0.2% metaphos-phoric acid 30 70 at high spped, filter through ca. 2 mm Hyflo Super-Cel coated on a suction funnel. Evaporate filtrate under reduced pressure at 50 to about 30 mL. Apply remaining solution to the SPE cartridge, wash with 20 mL water, elute with 10 mL MeOH. Evaporate eluate to dryness under reduced pressure, dissolve in 1 mL mobile phase, inject a 10 p.L aliquot. 

Hyfatol 16-95, Hyfatol 16-98. See Cetyl alcohol Hyfatol 18-95, Hyfatol 18-98. See Stearyl alcohol Hyfatol CS, Hyfatol CS/EP. See Cetearyl alcohol Hyflo Super-Cel . See Diatomaceous earth Hyflon MFA 620, Hyflon MPA 640. See Tetrafluoroethylene/perfluoromethylvinyl ether copolymer... 

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