Hydrolysis triglyceride, membrane reactor

Monoglyceride (MG) is one of the most important emulsifiers in food and pharmaceutical industries [280], MG is industrially produced by trans-esterification of fats and oils at high temperature with alkaline catalyst. The synthesis of MG by hydrolysis or glycerolysis of triglyceride (TG) with immobilized lipase attracted attention recently, because it has mild reaction conditions and avoids formation of side products. Silica and celite are often used as immobilization carriers [281], But the immobilized lipase particles are difficult to reuse due to adsorption of glycerol on this carriers [282], PVA/chitosan composite membrane reactor can be used for enzymatic processing of fats and oils. The immobilized activity of lipase was 2.64 IU/cm2 with a recovery of 24%. The membrane reactor was used in a two-phase system reaction to synthesize monoglyceride (MG) by hydrolysis of palm oil, which was reused for at least nine batches with yield of 32-50%. 

A model of a biphasic enzyme membrane reactor for the hydrolysis of triglycerides has been formulated according to the bond graph method of network thermodynamics, and the kinetics, the permeabilities of fatty acids and glycerides, the rates of inhibition of the immobilized enzyme, and the concentration of enzyme in a reaction zone are studied. 

The separation,purification and concentration of a thermosensitive bioactive compound from a lysate has been carried out combining UF, ion exchange and RO with significant cost reduction and productivity increase. Enzyme membrane reactors have been used for triglyceride enzymatic hydrolysis and product separation. Thermophi1ic,thermostable enzyme ultrafiltration membrane have been prepared, and used in high temperature lactose hydrolysis. 

Hydrolysis of olive oil triglycerides (lipase) Hydrophobic plate-and-frame membrane reactor Treatment of oils... 

Various membrane reactors have been proposed to allow recovery of products from microemulsions and permit to reuse the biocatalyst in various enzymatic processes such as hydrolysis of triglycerides [124,182], synthesis of esters [183,184], and peptide synthesis [65,185,186]. These reactors take advantage of the potential of synthetic semipermeable membranes, that usually are ultrafiltration membranes, to retain enzymes on the membrane and to recover products on the permeate side. The productivities as well as the enzyme stability in such reactors are usually high. 

As for flow patterns, counter-current flow is preferred, since it allows a higher efficiency in mass transfer rate, therefore increasing the overall reaction rate. For instance, the initial hydrolysis rate of triglycerides catalyzed by a lipase in a membrane two-phase reactor was 60% higher when a counter-cmrent mode was used, as compared to co-current mode [152]. 

We have been developing a membrane-type bloreactor for lipase-catalysed fat splitting (32). Using a stirred reactor, triglyceride hydrolysis was nearly complete within three hours (fig. 4). 

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