Hydrogen exchange spectrometry

Katta, V., Chait, B. T. Conformational-changes in proteins probed by hydrogen-exchange electrospray-ionization mass-spectrometry. Rapid Commun Mass Spectrom 1991, 5, 214-217. 

Zhang, Z. Smith, D.L. Determination of amide hydrogen exchange by mass spectrometry a new tool for protein structure elucidation. Protein Sd. 1993, 2, 522-531. 

Smith, D.L. Dharmasiri, K. Protein-ligand binding studied by amide hydrogen exchange and mass spectrometry. NATO ASI Ser. C 1998, 510, 45-58. 

Wang, L. Pan, H. Smith, D.L. Hydrogen exchange-mass spectrometry. Optimization of digestion conditions. Mol. Cell. Proteomics 2002, 1, 132-138. 

Dramatic advances in mass spectrometry and improvements in the various steps within the experimental hydrogen exchange procedures have resulted in the development of automated systems for high-throughput, high-resolution H/D exchange analysis [6, 8, 40-44]. 

Ehring H. Hydrogen exchange/ electrospray ionization mass spectrometry studies of structural features of proteins and protein/ protein interactions. Anal. Biochem. 1999, 267, 252-259. 

Wang Y., Gross M.L. Application of hydrogen exchange and electrospray ionization mass spectrometry in studies of protein structure and dynamics, in Applied Electrospray Mass Spectrometry (Practical Spectroscopy), ed. Pramanik BN, Ganguly AK, Gross ML 2002, pp 389-410. 

Hoofnagle A.N., Resing K.A., Ahn N.G. Protein analysis by hydrogen exchange mass spectrometry. Annu. Rev. Biophys. Biomol. Struct. 2003, 32, 1-25. 

Zhang Z., Post C.B., Smith D.L. Amide hydrogen exchange determined by mass spectrometry application to rabbit muscle aldolase. Biochemistry 1996, 35, 779—791. 

Wang L., Smith D.L. Downsizing improves sensitivity 100-fold for hydrogen exchange-mass spectrometry. Anal. Biochem. 2003, 314, 46-53. 

Wales, T.E. and Engen, J.R. (2006) Hydrogen exchange mass spectrometry for the analysis of protein dynamics. Mass Spectrom. Rev., 25, 158-70. 

Wang L, Lane LC, Smith DL (2001) Detecting structural changes in viral capsids by hydrogen exchange and mass spectrometry. Protein Sci 10 1234-1243... 

Buijs, J., Vera, C.C., Ayala, E., Steensma, E., Hakansson, P., and Oscarsson, S. (1999) Conformational stability of adsorbed insulin studied with mass spectrometry and hydrogen exchange. Analytical Chemistry, 71, 3219-3225. 

Mass spectrometry represents a complementary approach to NMR, providing faster analysis with high sequence coverage at lower protein amounts, although usually with reduced sequence resolution. MS enables detection at subpicomole sensitivity for hydrogen exchange measurements, which for many proteins is close to physiological concentration. 

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