Top-Down Hydrogen Exchange Mass Spectrometry

Igor A. Kaltashov, Rinat R. Abzalimov, Guanbo Wang, and Cedric E. Bobst Department of Chemistry, University of Massachusetts-Amherst, Amherst, MA, USA 

Hydrogen Exchange Mass Spectrometry of Proteins Fundamentals, Methods, and Applications, First Edition. Edited by David D. Weis. 

Extensive research efforts invested in the past several years in resolving these problems has resulted in a dramatic increase in the scope of proteins amenable to top-down HX-MS analysis, and this technique has now become a very valuable asset in the toolbox of experimental biophysics and structural biology. 

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Pan, J.X., Han, J., Borchers, C.H., Konermann, L. (2010) Characterizing short-lived protein folding intermediates by top-down hydrogen exchange mass spectrometry. Analytical Chemistry, 82 (20), 8591-8597. 

Top-Down Hydrogen Exchange Mass Spectrometry 157 Amide site... 

Pan, I, Han, I, Borchers, C.H., Konermann, L. (2009) Hydrogen/deuterium exchange mass spectrometry with top-down electron capture dissociation for characterizing structural transitions of a 17 kDa protein. /. Am. Chem. Soc., 131,12801-12808. 

J. Pan, J. Han, C. H. Borchers and L. Konermann. (2011) Conformer-Specific Hydrogen Exchange Analysis of AP(l-42) Oligomers by Top-Down Electron Capture Dissociation Mass Spectrometry, Analytical Chemistry, 83 (13), 5386-5393. 

Wang, G., Abzalimov, R.R., Bobst, C.E., Kaltashov, I.A. (2013) Conformer-specific characterization of non-native protein states using hydrogen exchange and top-down mass spectrometry. Proc Natl Acd Sci USA, 110 (50), 20087-20092. 

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