HYBRIDIZATION WITH NUCLEIC ACID PROBES properties

The interactions of nucleic acid materials with Ln have been largely studied through their luminescent properties. The known affinity of Ln " cations for the phosphate moiety has led to many investigations of the Ln interactions with nucleotides, covered in some detail by Evans [1]. The interaction of Ln with nucleic acids has been used to probe perturbations in the integrity of nucleic acid strands and as an assay of nucleic acid hybridization. Balcarova and Brabec [45] showed that the interaction of Tb with double-stranded DNA can be used to monitor guanine bases present in distorted double-stranded regions of DNA. The enhancement of distortions in double-stranded DNA appears to be restricted to certain modifiers of nucleic acid as there is no... 

In situ hybridization (ISH) consists of the application of hybridization techniques to intact cells which demonstrate genetic information within a morphologic context. This technology takes advantage of the hybridization properties of nucleic acids and offers a distinct technique to directly analyze sequence information in intact tissues. In essence, it combines cytogenetic techniques with molecular biology to probe gene alterations at molecular levels. Development of... 

The sensing events on the electronic transducers are designed to alter the interfacial properties at the transdncer/solution interface. Changes in the interfacial charge, capacitance, resistance, mass, thickness, and so forth would occur upon the hybridization of the analyte DNA with a probe nucleic acid, and upon stimulating the amplification ronte. Thus, any electronic transduction method that follows snch interfadal changes could be employed to detect the analysis of the DNA. 

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