Lipoprotein analysis human serum

Any discussion of serum lipids must ultimately lead to a consideration of the serum lipoproteins since it is in the form of lipoproteins that the lipids of the serum circulate. This volume presents two authoritative discussions on the subject of lipoproteins. One chapter discusses the analysis of human serum lipoproteins and emphasizes the possibilities inherent in the application of computer techniques to such analyses. The other chapter presents a thorough discussion of lipoprotein structure, metabolism, and catabolism. It touches on all aspects of lipoprotein metabolism. 

Detailed analysis of human serum lipoprotein will be one of the most valuable diagnostic tools, since it is considered to be directly related to the detection of fatal diseases, such as heart-attack, diabetes, various liver diseases and so on. However, there has not been a breakthrough method for it. Hara et al. 

The Analysis of Human Serum Lipoprotein Distributions Alicia M. Ewing, Norman K. Freeman, and Frank T. Lindgren... 

The frontal analysis technique has been used for the determination of enantioselective binding constants of chiral drugs such as warfarin, verapamil, nilvadipine, and semotidil with proteins such as bovine serum albumin (BSA), human serum albumin (HSA), and plasma lipoproteins (45-51). 

R2. Regnstrom, J., Strom, K., Moldeus, P., and Nilsson, J., Analysis of lipoprotein diene formation in human serum exposed to copper. Free Rad. Res. Comm. 19, 267-278 (1993). 

Human sera used in this method are obtained from normal male and female subjects, hyperlipidemia and other patients with various diseases after 12-16 hours of fasting. Serum is usually used for lipoprotein analysis by this technique. Plasma can be also used for this method. However, it should be noted that plasma might form fibrin during HPLC procedures, so that serum is preferable to plasma for samples in this technique. Sera are stored at 4 C, and analyzed within one week. Sera stored at -20 C can be used for analysis. But, in the case of hyper-... 

Fig. 9. Analysis of serum lipoproteins by monitoring A28O individual human subjects. Column G5000PW+G3000SW+G3000SW. Eluent 0.1 M Tris-HCl buffer (pH 7.4). Flow rate 1.0 ml/min. Loaded volume 20 pi. Sample the total lipoprotein fraction (d<1.210) from serum of (a), young female (b), old male (c), coronary heart disease (d), liver cirrhosis (e) and (f), hyperlipidemia. Peaks as in Fig. 5.

Film Analysis of Ultracentrifogal Runs at Solution Density 1.125 g./mL A typical analytical run on human serum at density 1.125 g./ml. is shown in Figure 9. Two additional components are determined in this analytical run a lipoprotein with a hydrated density of 1.05 g./ml., and a lipoprotein with a hydrated density of 1.075 g./ml. The 1.05 g./ml. lipoprotein is measured by making an enlarged tracing (as described above) utilizing the... 

R. Mallol, M. A. Rodriguez, J. Brezmes, L. Masana and X. Correig, Human Serum/Plasma Lipoprotein Analysis by NMR Application to the Study of Diabetic Dyslipidemia, Prog. Nucl. Magn. Reson. Spectrosc., 2013, 70, 1. 

Lipidomics profiling was used to determine the effects of plant sterols on lipid metabolism [142] since it is well known that plant sterol intervention could lead to reduction of both total cholesterol and LDL cholesterol in human [143]. In the study, two plant sterol-enriched yogurt drinks containing different fat content were given to healthy mildly hypercholesterolemic subjects for 4 weeks and then lipidomic analysis of serum samples were performed [142]. It was found that both drinks resulted in reduction of both total and LDL cholesterol levels [144]. In addition, the low-fat drink resulted in a reduction of the levels of several SM species. This reduction, which correlates well with the reduction of LDL cholesterol, could be due to the co-localization of SM and cholesterol on the surface layer of LDL lipoproteins. 

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