Human serum albumin chiral selectors

DK Lloyd, A Ahmed, F Pastore. A quantitative relationship between capacity factor and selector concentration in capillary electrophoresis and high-performance liquid chromatography evidence from the enantioselective resolution of benzoin using human serum albumin as chiral selector. Electrophoresis 18 958-964, 1997. 

A Ahmed, DK Lloyd. Effect of organic modifiers on retention and enantiomeric separations by capillary electrophoresis with human serum albumin as a chiral selector in solution. J Chromatogr A 766 237—244, 1997. 

Silica-base stationary phases have also been employed for enantiomeric separations in CEC [6,72-81]. In the initial work on chiral CEC, commercially available HPLC materials were utilized, including cyclodextrins [6,74,81] and protein-type selectors [73,75,80] such as human serum albumin [75] and ai-acid glycoprotein [73]. Fig. 4.9, for example, depicts the structure of a cyclodextrin-base stationary phase used in CEC and the separation of mephobarbital enantiomers by capillary LC and CEC in a capillary column packed with such a phase. The column operated in the CEC mode affords higher separation efficiency than in the capillary LC mode. Other enantiomeric selectors are also use in CEC, including the silica-linked or silica-coated macrocyclic antibiotics vancomycin [82,83] and teicoplanin [84], cyclodextrin-base polymer coated silicas [72,78], and weak anion-exchage type chiral phases [85]. Relatively high separation efficiency and excellent resolution for a variety of compounds have also been achieved using columns packed with naproxen-derived and Whelk-0 chiral stationary phases linked to 3 pm silica particles [79]. Fig. 4.10 shows the... 

Martmez-Pla J.J., Martm-Biosca Y, Sagrado S., Villanueva-Camanas R.M., Medina-Hernandez M.J., Chiral separation of bupivacaine enantiomers by capillary electrophoresis partial-filling technique with human serum albumin as chiral selector. J. Chromatogr. A, 1048, 111-118 (2004). 

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