Human ESCs

Because ESCs are pluripotent and can proliferate indefinitely, they may have an important potential role in cardiac regeneration. Although ethical issues involving the use of human ESCs has slowed research in several countries, including the United States, enthusiasm about their future clinical utilization remains high. 

Chen, T.S., et al., 2011. Enabling a robust scalable manufacturing process for therapeutic exosomes through oncogenic immortalization of human ESC-derived MSCs. J. Transl. Med. 9, 47-57. 

Nakano T, Ando S, Takata N, Kawada M, Mnguruma K, Sekiguchi K, Saito K, Yonemura S, Eirakn M, Sasai Y (2012) Selfformation of optic cups and storable stratified neural retina from human ESCs. Cell Stem Cell 10 771-785. 

Bovine wrists (trabecular bone) SDS + DNase and RNase treatments Human ESCs (cell line H9) NA Marcos-Campos et al. (2012)... 

Human ESCs have been shown to display significant differences to mouse ESCs they do not respond to LIF, instead relying on FGF and activin/nodal signalling. " Small molecules identified to allow the expansion of mouse ESCs... 

Hockemeyer D, Soldner F, Beard C, Gao Q, Mitalipova M, DeKelver RC, et al. Efficient targeting of expressed and silent genes in human ESCs and iPSCs using zinc-finger nucleases. Nat Biotechnol 2009 27 851—7. 

Embryonic stem cells (ESCs), aside from ethical and political debate, remain under scrutiny for the tendency of these cells to form teratomas upon implantation. However, this has not prevented the study of nonhuman ESCs for bone engineering in a hmited scope. Recently, increased expression of osteogenic markers was observed for human ESCs (hESCs) predifferentiated to osteoprogenitor cells and cultured on both 2D and 3D PLEA nanofibrous scaffolds. This indicates the potential of nanofibrous architecture to induce further osteogenic differentiation of hESCs (Smith et al. 2010). 

Mixed results utilizing TGF-Pl, 3 and BMPs indicate their effects are time dependent over the course of EB formation and chondrogenesis. Studies have shown TGF-Pl to be ineffective at inducing chondrogenesis in mouse and human ESCs [68]. However, TGF-P and parathyroid treatment following retinoic... 

Biomaterial Design for Human ESCs and iPSCs on Feeder-Free Culture toward Pharmaceutical... 

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