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HPAEC-PAD

Oligosaccharides were isolated from PMII by weak acid hydrolysis and separation by SEC and HPAEC-PAD. The isolated oligosaccharides were desalted, reduced and methylated. GC-MS analysis of the partially methylated alditol acetates has been used to reveal the structure of the oligosaccharides. [Pg.619]

The partially hydrolysed material was fractionated by size exclusion chromatography on a Bio Gel PIO (Bio Rad) column (2,6 x 90 cm) and eluted with 50 mM ammonium hydrogen carbonate at 20 ml/h and fractions of 3.2 ml each were collected. Fractions 45-70 were pooled and subjected to HPAEC-PAD for further separation. [Pg.619]

Figure 1. Separation of oligosaccharides from PMII by HPAEC-PAD. Figure 1. Separation of oligosaccharides from PMII by HPAEC-PAD.
Cordelia, C. B., Militao, J. S., Clement, M. C., and Cabrol-Bass, D. (2003b). Honey haracter-ization and adulteration detection by pattern recognition applied on HPAEC-PAD profiles. 1. Honey floral species characterization. /. Agric. Food Chem. 51,3234-3242. [Pg.125]

Recent applications of HPAEC-PAD are many and varied. A representative list includes quantitation of polyglucose metabolites in plasma of dialysis patients,148 analysis of heat-treated milk,149 carbohydrate content in lipopolysaccharides,150 phosphorylated sugars in tissue samples,151 composition of soybean meal,152 carbohydrate composition of recombinant modified tissue plasminogen activator,153 analysis of cyclization products from an enzyme reaction,154 carbohydrate content of glycoconjugate vaccines,155 and monitoring of patients with rheumatoid arthritis.156... [Pg.299]

Wong, K. S., Jane, J. -L. (1997). Quantitative analysis of debranched amylopectin by HPAEC-PAD with a postcolumn enzyme reactor. Journal of Liquid Chromatography, 20,297-310. [Pg.248]

Cataldi, T.R.I., NardieUo, D. (2003). Determination of free proUne and monosaccharides in wine samples by high-performance anion-exchange chromatography with pulsed amperometiic detection (HPAEC-PAD). J. Agric. Eood Chem., 51, 3737-3742. [Pg.247]

HPAEC-PAD High-performance anion-exchange chromatography-pulsed amperometric detection... [Pg.11]

HPAEC-PAD high-performance anion-exchange chromatography... [Pg.1414]

See other pages where HPAEC-PAD is mentioned: [Pg.7]    [Pg.35]    [Pg.275]    [Pg.277]    [Pg.620]    [Pg.621]    [Pg.299]    [Pg.299]    [Pg.299]    [Pg.231]    [Pg.231]    [Pg.231]    [Pg.239]    [Pg.257]    [Pg.122]    [Pg.225]    [Pg.25]    [Pg.213]    [Pg.315]    [Pg.105]    [Pg.121]    [Pg.65]    [Pg.65]    [Pg.67]    [Pg.67]    [Pg.67]    [Pg.69]    [Pg.69]    [Pg.71]    [Pg.71]    [Pg.72]    [Pg.73]    [Pg.86]    [Pg.1174]    [Pg.1197]   
See also in sourсe #XX -- [ Pg.225 ]

See also in sourсe #XX -- [ Pg.7 ]



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