How to test an HPLC instrument and its modules

An essential prerequisite for an accurate and precise analysis is well-functioning equipment. [Pg.39]

The simplest and safest method for an equipment check is a double injection with a known, stable sample, (control sample. System Suitability Test SST). If the resulting chromatograms are identical with a reference chromatogram which you obtained with the equipment in perfect condition - maybe after a scheduled master check - there is no need for further checks. You know that everything works well (method plus equipment). As criteria for the comparison, you could use retention time, peak area, peak height and asymmetry. Acceptable deviations are to be documented. [Pg.39]

If you have any deviation, you will have to check the status of the HPLC modules. Which tests are suitable [Pg.39]

HPLC module Measurement point Measurement Recommended limits/ comments [Pg.39]

Whole HPLC System Precision of peak area of isocratic — [Pg.39]

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