Homologous proteins testing

Homologous proteins Testing purified animal Data should be interpreted... 

Similar results were obtained with a different but less sensitive test system (76,102), utilizing 0.5 pg of I-labeled F(ab )2 fingments of IgG myeloma protein and rabbit antiidiotypic antiserum. Complexes were precipitated by goat anti-rabbit Fc. The ratio of the inhibitory capacity of homologous protein to nonspecific IgG exceeded 3 x 1(1 to I (102). 

Homologous proteins and/or systems Testing purified animal protein in the same species or, for monoclonal antibodies, testing an antibody directed against the receptor in the animal. Data should be interpreted with caution as the biological properties of the animal protein may differ from those of the human protein. 

Most of the studies with these alternative models were conducted in discovery research. However, several respondents had conducted pivotal regulatory toxicity studies with homologous proteins and animal disease models, either to allow products such as MAbs and cytokines to be tested in man, or in support of regulatory toxicity studies by clarifying a finding. Only one respondent had conducted pivotal regulatory toxicity studies with transgenic animals in order to take a MAb into man. 

It has been clear for some time that chemical shifts in proteins and nucleic acids depend upon structure in a reasonably regular way, so that, for example, the shifts in structurally homologous proteins are themselves homologous. It should be possible to exploit this information in structural studies, since many of the underlying physical interactions that lead to chemical shift dispersion are known, and there is now a sufficiently large number of assignments to allow useful empirical tests of these models to be carried out. 

C Lee. Testing homology modeling on mutant proteins Pi edictmg stiaictural and thermodynamic effects m the Ala98 Val mutants of T4 lysozyme. Folding Des 1 1-12, 1995. 

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