Homo-polynucleotide

Complexes formed between different homo-polynucleotides usually adopt the Watson-Crick base-pairing scheme as, for instance, poly(C) poly(G), poly(C) poly(I), poly(A) poly(U), poly(dA) poly(dT), etc. They are consequently right-handed double helices with antiparallel polynucleotide strands. 

Hereinafter, poly(X) denotes a homo-polynucleotide and X is the base symbol such as A (adenosine monophosphate), C (cytidine monophosphate), G (guanosine monophosphate), U(uridine monophosphate), I (inosine monophosphate), dA (deoxyadenosine monophosphate), etc. 

Table 6 (section 3.2.2.S) contains the pK values of homo-polynucleotides at room temperature as a function of ionic strength. Because of the relationship between pK, ionic strength and temperature, this additional table has been included. Because of the scarcity of the data in the literature this table is much shorter. 

If the gap of the nucleotide base stacks is really about 5 eV, the question of whether periodic (homo)polynucleotides could become intrinsic conductors because of the 0.2e charge shift between the sugar and base molecules seems to be legitimate, and will only be answered through even more accurate computations than are now possible. 

Another y9-(1 3)-D-glucan, named schizophyllan as an antitumor polysaccharide, has been obtained from Schizophylium commune [8,9]. Schizophyllan has similar chemical structure and triple-helix as lentinan [17], as shown in Figure 4. Since Sakurai and Shinkai were the first to find that schizophyllan may form a helical complex with single-stranded homo-polynucleotides [18], many works about preparing a complex of schizophyllan and... 

DHPLC (C2, L3, U1) uses a proprietary matched ion polynucleotide chromatography column devised by Transgenomics, Inc., in combination with an organic solvent and thermal denaturation (rather than an electrophoretic gel) to resolve heteroduplexes containing a suspected polymorphism from a polymorphism-free homoduplex. Homo- and heteroduplexes are visualized through UV absorbance readings of the eluent as it leaves the column. 

Sakurai K, Iguchi R, Mizu M et al (2003) Polysaccharide-polynucleotide complexes. Part 7. Hydrogen-ion and salt concentration dependence of complexation between schizophyllan and single-stranded homo RNAs. Bioorg Chem 31(3) 216-226... 

A homo-base quadruple has been actually observed for [poly(G)]4 and for [poly(I)l4 where the amino group in position 2 is lacking. It has the form depicted in Fig. 16.8. Since the glycosyl links are related by the fourfold helix axis, the polynucleotide strands are parallel to each other. 

The strong interactions between certain bivalent cations and the highly ordered polynucleotides such as DNA, certain synthetic duplex homo- and heteropolymers, and s-RNA have been the subject of much current interest. With this idea in mind, it is interesting to note that the steroidal diamine cyclobuxine-D (242) was shown to exert a profound effect on the stability of helical polynucleotides (DNA, s-RNA, and dAT dAT). The effect is biphasic in the sense that at low concentrations of the agent there is stabilisation of the native conformation, while at higher concentrations there is stabilisation of the denatured conformation. 

EC 2.7.7.7) an enzyme which catalyses the synthesis of DNA polynucleotide chains on a pre-existing DNA matrix (DNA replication). The precursors are the four 3 -deoxyribonucIeotide triphosphates. In vitro, the enzyme can also synthesize homo- and copolymers from triphosphates. The D.p. from different organisms have different specificities, some for single and others for double strands of DNA as primers. 

A. M. Michelson and C. Monny Polynucleotide analogues XII. Poly 5-homo-... 

Table 5 (section 3.2.2.4) contains melting temperatures of complexes between the same polynucleotide (homo-complexes). This table contains double, triple and four stranded complexes. Also included ate several polynucleotides which have no structure which is indicated as that... 

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