History enzyme kinetics

Applications of chemical kinetics to enzyme-catalyzed reactions soon followed. Because of the ease with which its progress could be monitored polarimetrically, enzyme hydrolysis of sucrose by invertase was a popular system for study. O Sullivan and Tompson (1890) concluded that the reaction obeyed the Law of Mass Action and in a paper entitled, Invertase A Contribution to the History of an Enzyme or Unorganized Ferment , they wrote [Enzymes] possess a life function without life. Is there anything [in their actions] which can be distinguished from ordinary chemical action ... 

A coating bearing one enzyme (papain) is produced on the surface of a glass pH electrode by the method previously introduced (co-crosslinking). The papain reaction decreases the pH, and the pH-activity variation gives an autocatalytic effect for pH values greater than the optimum under zero-order kinetics for the substrate (benzoyl arginine ethyl ester) the pH inside the membrane is studied as a function of the pH in the bulk solution in which the electrode is immersed. A hysteresis effect is observed and the enzyme reaction rate depends not only on the metabolite concentrations, but also on the history of the system. 

The results of treating wool with proteolytic enzymes will be unpredictable in the absence of detailed knowledge of (a) the processing history of the substrate and (b) how specific process conditions affect subsequent enzymatic treatments. To elucidate this point, the effects of adsorbed ionic species on enzyme/substrate adsorption and reaction kinetics were studied. ... 

The study of the effects of hydrogen ion equilibria on enzyme reactions has a long and distinguished history. It made contributions to the reliability of kinetic information, to our knowledge of protein stability and to the identification of essential catalytic groups and their reactivities. Sorensen (1912) made major contributions to the study of reactions of proteins. They were based on the great tradition of the Copenhagen school of physical... 

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