High Throughput Screening HTS Method

In order to increase throughput, the traditional shake-flask methodology has been transferred onto 96-well plate technology and the sample preparation is carried out using a robotic liquid handler. After equilibration of the solute in 

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The 3T3 NRU test may easily be performed under GLP conditions in contract research organizations (CROs) and a high-throughput screening (HTS) method was recently reported in the literature. The HTS method produced no false positives, although some false negatives were observed, suggesting that the standard 3T3 NRU test protocol remains necessary for the final selection of nonphototoxic compounds [42]. 

Paessens, A. Replication assay for detecting antiviral substances with a high throughput screening (HTS) method. Ger. Offen. DE 10311563, 2004 Chem. Abstr. 2004,141, 271533. 

High-throughput screening (HTS) is today the most commonplace method for identifying lead compounds that can be subsequently optimized to generate drug candidates. 

One of the established methods for finding hits is high-throughput screening (HTS). This is a powerful method enabling several thousands of compounds per day to be tested. However, there are drawbacks. In many cases the assay for the detection of a hit comprises coupled enzymatic reactions, and a test compound may falsely lead to positive results because of its interference with components of the enzyme cascade. Problems may arise further from the chemical substances that make up the compound depository which are tested in HTS. Especially for companies with historically grown collections of compounds, the quality of the chemicals is an uncontrolled parameter in the sense that it is not always known whether the compounds are still unmodified they may have decomposed, precipitated or formed aggregates (polymers). 

The novel concept of High-Throughput Screening (HTS) assay, that has gained widespread popularity over the last two decades, is a valid approach to drug discovery and has become a standard method for drug discovery in the pharmaceutical industry [34],... 

Advances in combinatorial chemistry methods to increase the number of compounds for high throughput screening (HTS), and... 

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