High-throughput colorimetric screen

Using the sialosides obtained, a 96-well plate based coupled enzymatic assay for high-throughput colorimetric screening of structures that can be recognized by different sialidases was developed (Scheme 7) (75). Briefly, individual sialosides are incubated with an appropriate amount of a certain sialidase and an excess amount of exogalactosidase or hexosaminidase. If the... 

Seven different bacterial sialidases, including six commercially available sialidases from Arthrobacter ureafaciens, Clostridium perfingens. Streptococcus sp. IID, Vibrio cholera. Salmonella typhimurium, and Streptococcus pneumoniae, as well as PmSTl which also possesses sialidase activity (20), were used as model systems to test the application of the sialoside library and the 96-well plate based high-throughput colorimetric screening method. 

Ncokazi, K. K., and Egan, T. J. (2005). A colorimetric high-throughput beta-hematin inhibition screening assay for use in the search for antimalarial compounds. Anal. Biochem. 338, 306-319. 

Wang D.Wang J, Wang B,Yu H. A new and efficient colorimetric high-throughput screening method for triacylglycerol lipase directed evolution. Mol Catal B Enzym 2012 82 18-23. 

UV/visual spectroscopy based on colorimetric assays is regularly used in low-throughput screens based on microtiter plates with a low density of wells (usually 96 wells per plate). Further increases in throughput by using high-density sample carriers has proven dilBcult for UV/visual spectroscopy detection systems. For most such applications, sample volume and thus... 

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