Low-Throughput Screening

Screening in 96- or 384-well plate formats allows precise fitness measurements. The accuracy of the detection system, kinetic assays (in contrast to end point assays), the possibility to normalize activity values (e. g., using measured cell concentrations), and better control over cell growth and protein expression contribute to the improved 

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UV/visual spectroscopy based on colorimetric assays is regularly used in low-throughput screens based on microtiter plates with a low density of wells (usually 96 wells per plate). Further increases in throughput by using high-density sample carriers has proven dilBcult for UV/visual spectroscopy detection systems. For most such applications, sample volume and thus... 

In recent years, iPSCs have become an attractive alternative that incorporate the advantageous feature of immortalized cell lines such as their ability to divide and expand indefinitely, allowing for scale-up. iPSCs also hold the promise of having the capability to differentiate into many different cell types with the phenotype of a primary cell. As an early example of this potential, particularly when compared to immortalized cell lines, Haggarty and colleagues recently conducted a low-throughput screen on a library of approximately 1500 compounds... 

Felcht reports that the testing of industrial-scale processes can be performed with low expenditure by using micro reactors, since this should result in a faster time to market of the development [137]. He also sees uses for micro reactors at the laboratory scale as a means of high-throughput screening and model examinations such as fast determination of reaction kinetics. 

Yaccato K, Hagemeyer A, Lesik A, Volpe A, Weinberg WH. 2004. High throughput screening of low temperature SCR and SCD de-NOx catalysts in scanning mass spectrometer. Top Catal 30/31 127-132. 

Considering that PAMPA is a high-throughput screening method, the errors are low enough to encourage the use of the method to study mechanistic properties, as our group at pION has done since 1997. 

Yang, Y. Z., Babiak, P. and Reymond, J. L. (2006b). Low background FRET-substrates for lipases and esterases suitable for high-throughput screening under basic (pH 11) conditions. Org. Biomol. Chem. 4,1746-1754. 

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