Analysis high throughput

McDougall G, Martinussen I and Stewart D. 2008. Towards fruitful metabolomics high throughput analyses of polyphenol composition in berries using direct infusion mass spectrometry. J Chromatogr B 871(2) 362-369. 

Utilizing Microparallel Liquid Chromatography for High-Throughput Analyses in the Pharmaceutical Industry... 

Although this book focuses on high-throughput analyses in the pharmaceutical industry, applications in environmental analysis are closely related. The same technologies are applicable to both fields. Pharmaceuticals have been monitored as pollutants in surface water, soil, food, and human plasma. In environmental applications, as many as 30 to 40 analytes have been monitored simultaneously. 

Novel Instrument Configurations for High-Throughputs Analyses... 

The most prominent field of applications for microchip—MS concerns identification and analysis of large molecules in the field of proteomics according to the reduced separation time compared to conventional approaches such as gel-based methods for protein analysis. High-throughput analyses, with lower contamination and disposability, are other features of microfabricated devices that allow the fast screening of proteomic samples in the clinical field. Applications also include the analysis of low-molecular-weight compounds such as peptides or pharmaceutical samples. 

Shen, D., Chang, H.R., Chen, Z., He, J., Lonsberry, V., Elshimali, Y., Chia, D., Seligson, D., Goodghck, L., Nelson, S.F., and J.A. Gornbein, 2005, Loss of annexin Al expression in human breast cancer detected by multiple high-throughput analyses. Biochem Biophys Res Commun. 326(1) 218-27. 

This example shows progress in the miniaturizing of the experimental approach and acceleration of rhizodeposit characterization, which is a first step toward time-resolved, high-throughput analyses to disclose plant physiological and microbial processes in the rhizosphere. 

Ishii N, Nakahigashi K, Baba T et al (2007) Multiple high-throughput analyses monitor the response of E. coli to perturbations. Science 316 593-597... 

DNA sequence analyses and/or to analyze oligonucleotides, DNA-restriction fragments, amino acids or PCR products. Many hundred thousand analytical data points can be accumulated per day using commercially available instruments [55], Therefore chiral modification of such techniques, if successful, should allow for the first time super-high-throughput analyses of enantiomeric purity (ee) [51]. 

Campbell CT, Kim G (2007) SPR microscopy and its applications to high-throughput analyses of biomolecular binding events and their kinetics. Biomaterials 28 2380-2392... 

These new omics technologies will also enable us to efficiently identify novel compounds of value to both the Agrochemical and Pharmaceutical industries. It is of crucial importance that the data that results from high throughput analyses of the genome, transcriptome, proteome and metabolome of crop plants and foeir relatives is archived in an appropriately structured and indexed manner. This will enable it to be fully integrated and data-mined over an extensive period as our ability to formulate increasingly sophisticated queries on these data sets develops. 

The small peak volumes and resolution demands from high-throughput analyses require faster data collection rates for accurate results. Adequate detector time constants should also be used. 

FIGURE 14.2 High throughput analyses of samples A and B using LC-MS vs. SFC-MS. 

Quadrupole mass filters are one of the most common and cost effective mass analyzers. Although they have limited mass resolution (Table 10.2) and are less sensitive than other mass analyzers, they are durable and suitable for high-throughput analyses. To perform a tandem mass analysis (MS/MS), a triple quadrupole MS is used in which three quadrupoles are placed in series to select, fragment, and analyze ions of interest. Quadrupole ion-trapping devices such as linear 2D ion traps are... 

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