High-throughput analysis technologies

The rapid development of combinatorial screening methods has been accompanied by the development of ever more efficient high-throughput analysis technologies. These not only enable analysis of catalytic activity but also the determination of enantiomeric excess [2, 21]. Taking these developments together, research in this field can be expected to yield highly active and selective catalysts with structures that could have not been predicted by conventional means. 

The following protocol is based on the method of FitzGerald et al. (2005), who used the technology to develop aluminum oxide-based biochips for high-throughput analysis. 

Taylor R.L., Grebe S.K., and Singh R., High throughput analysis of 25-hydroxyvitamins D2 and D3 by LC-MS/ MS using automated online extraction. Application poster, Mayo Clinic and Cohesive Technologies. 

Proteomics involves the systematic analysis of proteins to determine their identity, quantity, and function (Soloviev et al., 2004). Until recently, the study of proteins focused on individual proteins using various established techniques such as gel-electrophoresis and chromatography. Hie advent of high-throughput automated technologies is now facilitating the move toward simultaneous analysis of all the proteins in a defined protein population (Soloviev et al., 2004, see also Jones and Warren, 2006). 

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