High screening assay

Table 8.2 shows the range of possible 71 values, with comments on their meaning in terms of high-throughput screening assays. 

Macarron R, Hertzberg RP (2002) Design and implementation of high throughput screening assays. Methods Mol Biol 190 1-29... 

Zhang JH, Chung TD, Oldenburg KR (1999) A simple statistical parameter for use in evaluation and validation of high throughput screening assays. J Biomol Screen 4(2) 67-73... 

Andexer, J., Guterl, J.-K., Pohl, M. and Eggert, T. (2006) A high-throughput screening assay for hydroxynitrile lyase activity. Chemical Communications, 4201 4203. 

Krammer, B., Rumbold, K., Tschemmernegg, M. et al. (2007) A novel screening assay for hydroxynitrile lyases suitable for high-throughput screening. Journal of Biotechnology, 129, 151-161. 

Macarron, R., and Hertzberg, R. P. (2002). Design and Implementation of High Throughput Screening Assays. Humana Press, Totowa, NJ. 

Compared to in vivo studies, the Caco-2 model substantially increases the speed at which absorption potential can be estimated and reduces the amount of drug substance needed. However, manually performed assays are still too slow and labor intensive compared to biological high-throughput screening assays. Caco-2 cells take about 3 weeks to form monolayers of fully differentiated cells. At this point, Caco-2 monolayers are used to evaluate absorption potential under a variety of permeability protocols. In order to further expedite the process of absorption potential assessment, efforts have been made to increase the throughput of Caco-2 transport experiments. 

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