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High Resolution Melting Analysis

Krypuy M, Newnham GM, Thomas DM, et al. High resolution melting analysis for the rapid and sensitive detection of mutations in clinical samples KRAS codon 12 and 13 mutations in nonsmall cell lung cancer. BMC Cancer. 2006 6 295. [Pg.56]

Hill CE, Duncan A, Wirth D, et al. Detection and identification of cytochrome P-450 2C9 alleles 1, 2, and 3 by high-resolution melting curve analysis of PCR amplicons. Am J Clin Pathol 2006 125(4) 584-91. [Pg.41]

Willmore-Payne C, Holden JA, Chadwick BE, Layfield LJ. Detection of c-kit exons 11- and 17-activating mutations in testicular seminomas by high-resolution melting amplicon analysis. Mod Pathol. 2006-,19 1164. [Pg.660]

In a semi-crystalline polymer or in a polymer that contains crystalline domains, it is necessary to break apart all die crystalline domains for a true representative high resolution NMR analysis. This can be achieved by either addition of solvent or acquisition of data at temperatures close to the melting temperature of the crystalline domains. At temperatures above the melting point... [Pg.40]

The study of carbon electrodeposition from carbonate and halide- carbonate melts was devoted a cycle of works [1 - 6]. It was found that under certain conditions carbon solid phase was formed on cathode with 100 % current yield. The questions of product morphology, structure and dispersity had remained not opened because of absence at that time analysis technical equipment with high-resolution. Different schools of the electrochemists offered various mechanisms of carbon phase formation ... [Pg.459]

All of the calystegines isolated to date have been obtained as amorphous white solids and melting points have therefore not been reported. Structures have been determined by spectroscopic methods, in particular H and 1SC NMR spectroscopy and high-resolution mass spectrometry. Additional confirmatory evidence has been obtained from analysis of the alkaloids as trimethylsilyl ether derivatives by gas chromatography - mass spectrometry (Section 4.4). In certain cases the structures and absolute configurations have been confirmed by synthesis (Section 6). [Pg.310]

Wfllmore C, Holden JA, Zhou L, Tripp S, Wittwer CT, Layfield LJ. Detection of c-kit activating mutations in gastrointestinal stromal tumors by high-resolution amplicon melting analysis. Am J CHn Path 2004 122 206-16. [Pg.1448]

Wittwer CT> Reed GH, Gundry CN, Vandersteen JG, Pryor RJ. High-resolution genotyping by amplicon melting analysis using LCGreen. Clin Chem 2003 49 853-60. [Pg.1449]

Zhou L, Vandersteen J, Wang L, Fuller T, Taylor M, Palais B, Wittwer CT. High-resolution DNA melting curve analysis to establish HLA genotypic identity. Tissue Antigens 2004 64 156-64. [Pg.1449]

High-resolution proton DQ MAS NMR is used as a new technique that is capable of revealing complex motional processes in entangled polymer melts. Theoretical analysis shows the connection of quantities relating anisotropic polymer dynamics to data obtained from our DQ-MAS NMR experiment. With this technique, dynamic chain ordering as well as scaling laws consistent with the reptation model was previously observed for polybutadiene (PB). [Pg.259]

In the opinion of the authors this equation may be considered as the Arrhenius-parallel emerging at high temperatures. Noteworthy is the difference from the classical Arrhenius equation recalled in the introduction. There is an extensive evidence for the crossover from the VET- to the Arrhenius-type behavior on heating above the melting temperature. " However, the analysis of high resolution experimental data for glass forming liquids indicate also the... [Pg.100]

MHz high resolution solid-state NMR spectroscopy [53, 54]. All the samples used for this NMR study are random copolymers and not mixtures of random copolymers as confirmed by the solution NMR sequence analysis and by DSC melting-point measurements [43, 54]. Before taking NMR measurements, possible effects of the thermal history and recrystallization are avoided as mentioned above. [Pg.780]

Wittwer, C. T. Gundry, C. High resolution genotyping of nucleic acid polymorphisms using melting curve analysis. Eur. Pat. Appl. EP 1362928, 2003 Chem. Abstr. 2003,139, 376184. [Pg.292]

High-resolution TEM only probes a very small volume, which may or may not be representative of the total composite. Moreover sample preparation for TEM analysis is difficult and time-consuming. Therefore, the method is too costly for routine characterization of nanoeomposites. As a result researchers are now using melt rheology as a complementary method to analyse the dis-persion/exfoliation in polymer lay nanoeomposites. Several authors found that the shear thinning index, n, gives a semi-quantitative measure of the degree of exfoliation. [Pg.399]


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High-resolution analyses

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