High performance liquid screening

Tian, Q. et al., Screening for anthocyanins using high-performance liquid chromatography coupled to electrospray ionization tandem mass spectrometry with precursor-ion analysis, product-ion analysis, common-neutral-loss analysis, and selected reaction monitoring, J. Chromatogr. A, 1091, 72, 2005. 

Bogusz, M., Franke, J. P., de Zeeuw, R. A., and Erkens, M., An overview of the standardization of chromatographic methods for screening analysis in toxicology by means of retention indices and secondary standards. Part II. High performance liquid chromatography, Fresenius ]. Anal. Chem., 347, 73, 1993. 

The use of direct UV spectrophotometry to measure sample concentrations in pharmaceutical research is uncommon, presumably due to the prevalence and attractiveness of high-performance liquid chromatography (HPLC) and liquid chromatography/mass spectrometry (LC/MS) methods. Consequently, most researchers are unfamiliar with the value of UV detection, mainly that it is generally much faster than other methods - a very important asset in high-throughput screening. 

Chong BE, Lubman DM, Miller FR, et al. Rapid screening of protein profiles of human breast cancer cell lines using non-porous reversed-phase high performance liquid chromatography separation with matrix-assisted laser desorption/ionization time-of-flight mass spectral analysis. Rapid Commun. Mass Spectrom. 1999 13 1808-1812. 

Consider one small molecule, phenylalanine. It is an essential amino acid in our diet and is important in protein synthesis (a component of protein), as well as a precursor to tyrosine and neurotransmitters. Phenylalanine is one of several amino acids that are measured in a variety of clinical methods, which include immunoassay, fluorometry, high performance liquid chromatography (HPLC see Section 4.1.2) and most recently MS/MS (see Chapter 3). Historically, screening labs utilized immunoassays or fluorimetric analysis. Diagnostic metabolic labs used the amino acid analyzer, which was a form of HPLC. Most recently, the tandem mass spectrometer has been used extensively in screening labs to analyze amino acids or in diagnostic labs as a universal detector for GC and LC techniques. Why did MS/MS replace older technological systems The answer to this question lies in the power of mass spectrometer. 

TLC is a screening method a small percentage (5 to 10%) of the samples should be confirmed by a more sophisticated analytical method such as gas chromatography or high performance liquid chromatography... 

L. Almela, J.A. Femandez-Lopez and M.J. Roca, High-performance liquid chromatographic screening of chlorophyll derivatives produced during fruit storage. J. Chromatogr.A 870 (2000) 483 189. 

Kuronen P, Volin P, Laitalainen T. 1998. Reversed-phase high-performance liquid chromatographic screening method for serum steroids using retention index and diode-array detection. J Chromatogr B 718(2) 211-224. 

Tournel G, Houdret N, Hedouin V, Deveaux M, Gosset D, Lhermitte M. 2001. High-performance liquid chromatographic method to screen and quantitate seven selective serotonin reuptake inhibitors in human serum. J Chromatogr B Biomed Sci Appl 761(2) 147-158. 

Schmeer K, Sauter T, Schmid J. 1997. Rapid screening of sal-butamol in plasma by column-switching high-performance liquid chromatography-electrospray mass spectrometry. J Chromatogr A 777 67. 

X. Xu, A.M. van der Craats and P.C.A.M. de Bmyn, Highly sensitive screening method for nitroaromatic, nitramines and nitrate ester explosives by high performance liquid chromatography — atmospheric pressure ionization — mass spectrometry (HPLC-APl-MS) in forensic applications , J. Forensic Sci., 49 No. 6 (2004) 1171-1180. 

Krahn M, Ylitalo G, Buzitis J, et al. 1993. Rapid high-performance liquid-chromatographic methods that screen for aromatic-compounds in environmental-samples. Journal of Chromatography 642(1-2) 15-32. 

Dohta, Y, Yamashita, T, Horiike, S., Nakamura, T. and Kukami, T. (2007) A system for logD screening of 96-weIl plates using a water-plug aspiration/ injection method combined with high-performance liquid chromatography-mass spectrometry. Analytical Chemistry, 79, 8312-8315. 

Khasawneh MA, Vallano PT, Remcho VT. Affinity screening by packed capillary high performance liquid chromatography using molecular imprinted sorhents. II. Covalent imprinted polymers. J Chromatogr A 2001 922 87-97. 

Tolonen, A. and Uusitalo, L, Fast screening method for the analysis of total flavonoid content in plants and foodstuffs by high-performance liquid chromatography/electrospray ionization time-of-flight mass spectrometry with polarity switching. Rapid Commun. Mass Spectrom., 18, 3113, 2004. 

Shakya, R., Navarre, D. A. (2006). Rapid screening of ascorbic acid, glycoalkaloids, and phenolics in potato using high-performance liquid chromatography. J. Agric. Food Chem., 54, 5253-5260. 

V-Nitrosodiethanolamine can be determined in air and bulk process samples. Air samples are collected on glass fibre filters, extracted with 2-propanol and analysed by gas chromatography with thermal energy analyser detection. The limit of detection is 200 ng per sample (0.42 pg/m ). Bulk samples can be screened for 7V-nitrosodiethanol-amine by high-performance liquid chromatography (HPLC) with ultraviolet detection (Occupational Safety and Health Administration, 1990). 

Sumi S, Kidouchi K, Ohba S et al. Automated screening system for purine and pyrimidine metabolism disorders using high performance liquid chromatography. J Chromatogr B Biomed Sci Appl 1995 672 233-239. 

Lai CC, Tsai CH, Tsai FJ, Wu JY, Lin WD, Lee CC (2002) Rapid screening assay of congenital adrenal hyperplasia by measuring 17 alpha-hydroxyprogesterone with high-performance liquid chromatography/electrospray ionization tandem mass spectrometry from dried blood spots. J Clin Lab Anal 16 20-25... 

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