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High performance liquid purification

Purification of poloxamers has been extensively investigated due to their use in medical applications, the intention often being to remove potentially toxic components. Supercritical fluid fractionation and liquid fractionation have been used successfully to remove low-molecular weight impurities and antioxidants from poloxamers. Gel filtration, high-performance liquid chromatography (HPLC), and ultrafiltration through membranes are among the other techniques examined [5]. [Pg.768]

Branchini, B. R., and Rollins, C. B. (1989). High-performance liquid chromatography-based purification of firefly luciferases. Photochem. Photobiol. 50 679-684. [Pg.384]

Although saponification was found to be unnecessary for the separation and quantification of carotenoids from leafy vegetables by high performance liquid chromatography (HPLC) or open column chromatography (OCC), saponification is usually employed to clean the extract when subsequent purification steps are required such as for nuclear magnetic resonance (NMR) spectroscopy and production of standards from natural sources. [Pg.452]

Mitcham, E.J., Gross, K.C., and Wasserman, B.P. (1991) Synthesis of uridinediphospho-[U- C]-D-galacturonic acid by enzyme particulate fractions and purification via high performance liquid chromatography. Phytochem.Anal. 2 112-115. [Pg.124]

Kroeff, E. P., Owens, R. A., Campbell, E. L., Johnson, R. D., and Marks, R. I., Production scale purification of synthetic human insulin by reversed-phase high-performance liquid chromatography, /. Chromatogr., 461, 45, 1989. [Pg.125]

Warren, W. and Vella, G., Analysis and purification of synthetic oligonucleotides by high-performance liquid chromatography, in Oligonucleotide Synthesis Protocols, Agrawal, S., Ed., Humana Press, Totowa, NJ, 1993, 235. [Pg.126]

Titani, K., Sasagawa, T., Resing, K., and Walsh, K. A., A simple and rapid purification of commercial trypsin and chymostrypsin by reverse-phase high-performance liquid chromatography, Anal. Biochem., 123, 408, 1982. [Pg.198]

Matejicek D, Houserova P, Kuban V (2007) Combined isolation and purification procedures prior to the high-performance liquid chromatographic-ion-trap tandem mass spectrometric determination of estrogens and their conjugates in river sediments. J Chromatogr A 1171 (l-2) 80-89... [Pg.45]

Materials required Autoclave, high performance liquid chromatograph, methanol, 2N HC1, pollen, Millipore UF Plus water purification system. [Pg.213]

Procedure Flavonoids are then further purified with 2 ml of methanolic HC1 (2 N), followed by centrifugation (2 min, 15 600 g), hydrolyzation of 150 il of suspension in an autoclave (15 min, 120 C). A reverse osmosis-Millipore UF Plus water purification system is used in high performance liquid chromatography (HPLC) with an autosampler. After injections of 5 pg of samples, the mobile phases flow at a rate of 1 ml/minute with isocratic elution in a column at 30 C. [Pg.213]

Wenk, S. O. and J. Kruip (2000) Novel, rapid purification of the membrane protein photosystem I by high-performance liquid chromatography on porous materials. J.Chromatogr. BBiomed SciAppl., 737 131-142... [Pg.179]

Shirai Y, Ono Y, Akimoto K (2000) Simultaneous determination of deoxynivalenol and nivalenol in grain by high performance liquid chromatography with multifunctional clean up column for purification. Res Rep Animal Feed 26 1-9... [Pg.434]

J Rivier, R McClintock, R Galyean, H Anderson. Reversed-phase high-performance liquid chromatography preparative purification of synthetic peptides. J Chromatog 288, 303, 1984. [Pg.257]

R. Bermejo, E.M. Talavera and J.M. Alvarez-Pez, Chromatographic purification and characterization of B-phycoerythrin of Porphyridium cruentum. Semipreparative high-performance liquid chromatographic separation and characterization of its subunits. J. Chromatogr.A 917 (2001) 135-145. [Pg.366]

K.J. Fountain, M. Gilar, Y. Budman and J.C. Gebler, Purification of dye-labeled oligonucleotides by ion-pair reversed-phase high-performance liquid chromatography. J. Chromatogr.B, 783 (2003) 61-72. [Pg.561]

M. Chen, D. Moir, F.M. Benoit and C. Kubwabo, Purification and identification of several sulphonated azo dyes using reversed-phase preparative high-performance liquid chromatography. J. Chromatogr.A, 825 (1998) 37-44. [Pg.570]

Analytical methods for determining disulfoton in environmental samples are reported in Table 6-2. The steps included in the methods are solvent extraction, purification and fractionation, and gas chromatographic analysis. Other analytical techniques, including capillary gas chromatography with mass selective detection (Stan 1989), high-performance liquid chromatography with either mass spectrometric (MS) or MS-MS detection (Betowski and Jones 1988), have been used to determine disulfoton in environmental samples. [Pg.160]

Burchiel S. W Billman, J. R and Alber, T. R. (1984) Rapid and efficient purification of mouse monoclonal antibodies from ascites fluid using high performance liquid chromatography. J. Immunol. Methods 69, 33-42. [Pg.22]

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See also in sourсe #XX -- [ Pg.102 ]



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