High-performance liquid library characterization

Zeng, L. Burton, L. Yung, K. Shushan, B. Kassel, D. B. 1998b. Automated analytical/preparative high-performance liquid chromatography-mass spectrometry system for the rapid characterization and purification of compound libraries. /. Chromatogr. A, 794,3-13. 

As for all synthetic products to be tested in biological systems, a careful analytical characterization of peptide libraries is crucial in order to confirm their identity and establish their quality. Compared to individual peptides, however, the analysis of peptide libraries is complicated due to the fact that the peptides are either bound to a solid support or arranged in highly complex mixtures. This poses certain restrictions on which analytical methods can be used to characterize combinatorial libraries. For example, analytical methods that are based on the separation of product components, such as high performance liquid chromatography (HPLC) and capillary electrophoresis (CE), are only of limited use for the analysis of peptide libraries, in particular of those made up of complex nnixtures (>100 peptides per mixture). The analytical methods beneficially applicable to peptide libraries include amino acid analysis, mass spectrometry, and sequencing. 

Wu, J.T. Zeng, H. Deng, Y. Unger, S.E. Automated Analytical/Preparative High-performance Liquid Chromatography-Mass Spectrometry System for the Rapid Characterization and Purification of Compound Libraries, Rapid Commun. Mass Spectrom. 15, 1113-1119 (2001). 

The oxygen heterocyclic compounds present in the nonvolatile residue of citrus essential oils has also been extensively investigated by means of high-performance liquid chromatography/ atmospheric pressure ionization mass spectrometry (HPLC/APl/MS) [99]. The mass spectra obtained at different voltages of the sample cone have been used to build a library. Citrus essential oils have been analyzed with this system, using an optimized NP-HPLC method, and the mass spectra were compared with those of the laboratory-constructed library. This approach allowed the rapid identification and characterization of oxygen heterocyclic compounds of citrus oils, the detection of some minor components for the first time in some oils, and also the detection of authenticity and possible adulteration. 

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