High-performance liquid chromatography with hydride generation atomic absorption

High performance liquid chromatography coupled with hydride generation-atomic absorption spectrometry has been used for the determination of arsenic species in non saline water samples [265],... 

Basic techniques for speciation analysis are typically composed of a succession of analytical steps, e.g. extraction either with organic solvents (e.g. toluene, dichloromethane) or different acids (e.g. acetic or hydrochloric acid), derivatisa-tion procedures (e.g. hydride generation, Grignard reactions), separation (gas chromatography (GC) or high-performance liquid chromatography (HPLC)), and detection by a wide variety of methods, e.g. atomic absorption spectrometry (AAS), mass spectrometry (MS), flame photometric detection (FPD), electron capture detection (ECD), etc. Each of these steps includes specific sources of error which have to be evaluated. 

M. A. Lopez, M. M. Gomez, C. Camara, Determination of six arsenic species by high-performance liquid chromatography - hydride generation - atomic absorption spectrometry with on-line thermo-oxidation, Fresenius J. Anal. Chem, 346 (1993), 643-647. 

Figure 3.40 Schematic of an LC-HGAAS system (Reprinted from Analytica Chimica Acta, Niedzielski, P. The new concept of hyphenated analytical system Simultaneous determination of inorganic arsenic(lll), arsenic(V), selenium(IV) and selenium(VI) by high performance liquid chromatography-hydride generation-(fast sequential) atomic absorption spectrometry during single analysis, 55 (1-2), 199-206 T Copyright 2005 with permission from Elsevier).

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