High performance liquid chromatography resins

New types of ion exchange resins have also been developed to meet the specific needs of high-performance liquid chromatography (HPLC) (Chapter 8). These include pellicular resins and microparticle packings (e.g. the Aminex-type resins produced by Bio-Rad). A review of the care, use and application of the various ion exchange packings available for HPLC is given in Ref. 19. 

High-ortho novolac resins, 379-381 High-performance liquid chromatography, 162... 

Lloyd, L Warner, FP, Preparative High-Performance Liquid Chromatography on a Unique High-Speed Macroporous Resin, Journal of Chromatography 512, 365, 1990. 

Yang, Y.-B., Harrison, K., and Kindsvater, J., Characterization of a novel stationary phase derived from a hydrophilic polystyrene-based resin for protein cation-exchange high-performance liquid chromatography, /. Chromatogr. A, 723, 1, 1996. 

G.A. van der Doelen, K.J. van den Berg, J.J. Boon, N. Shibayama, E.R. de la Rie, W.J.L. Genuit, Analysis of fresh triterpenoid resins and aged triterpenoid varnishes by high performance liquid chromatography atmospheric pressure chemical ionisation (tandem) mass spectrometry, Journal of Chromatography A, 809, 21 37 (1998). 

In addition to GC/MS, high performance liquid chromatography (HPLC/MS) has been used to analyse natural resins in ancient samples, particularly for paint varnishes containing mastic and dammar resins [34]. A partial limitation of chromatographic techniques is that they do not permit the analysis of the polymeric fraction or insoluble fraction that may be present in the native resins or formed in the course of ageing. Techniques based on the direct introduction of the sample in the mass spectrometer such as direct temperature resolved mass spectrometry (DTMS), direct exposure mass spectrometry (DE-MS) and direct inlet mass spectrometry (DI-MS), and on analytical pyrolysis (Py-GC/MS), have been employed as complementary techniques to obtain preliminary information on the... 

HPLC) for phenolic acids analysis. When procedure (ii) was applied, the ion-exchange resin was separated from the methanol phase and eluted with three 40 ml aliquots of 80% methanol. The resin bead eluates were evaporated to dryness and subjected to spectrophotometry (Shimadzu UV 160 spectrophotometer) for total phenolics and high-performance liquid chromatography (HPLC) for phenolic acids analysis. 

Dimethylamine (109), putrescine (111), and spermidine (110), isolated from various insects (Table VIII), were obtained as p,p -nitrophenylazobenzoyl, p-phenylazobenzenesulfonyl, and I-dimethylaminonaphthalene-5-sulfonyl (dan-syl) derivatives and picrates or were detected by high-performance liquid chromatography (HPLC) using the ion-exchange resin (106,343). V,V-Dimethyl-3-phenylethylamine (131) from spiders of the genus Sclerobunus (Table VIII) has been identified by mass spectral comparison with a synthetic sample (117). 

Kurosu, Y., Kawasaki, H., Chen, X.-C., Amano, Y., Fang, Y.-I., Isobe, T., and Okuyama, T., Comparison of retention times of polypeptides in reversed phase high performance liquid chromatography on polystyrene resin and on alkyl bonded silica, Bunseki Kagaku, 33, E301, 1984. 

Comparative assessments of purification protocols are generally difficult, and wide ranges of activities have been reported. ACV synthetases have been assayed by either employing a peptide adsorption resin (Porapak) [29,48] or high performance liquid chromatography (HPLC) detection of the derivatized tripeptide [26,27], The adsorption assay, employing radiolabeled amino acids, has been... 

Upon completion of the synthesis, the peptide was cleaved from the resin by treatment with liquid hydrofluoric acid (HF) for 45 minutes at 0°C. The HF was evaporated and the peptide treated with aqueous acetic acid and lyophilized. The crude peptide was then purified by high performance liquid chromatography (HPLC) on a C18 column, eluting with a mixture of acetonitrile and 0.1% TFA in water. Purified fractions (homogeneous by UV and TLC analysis) were combined and lyophilized. Analytical HPLC was used to determine the purity of the final product peptides synthesized was at least 98% pure. 

Arsenic species were preconcentrated on Zipax, a pellicular anion exchange material and separated on a column packed with high performance liquid chromatography grade strong anion exchange resin, then continuously reduced with sodium tetrahydroborate and detected by atomic absorption spectrometiy. Detection limits were 2ng for arsenite, arsenate and monomethylarsinate and lng for dimethylarsonate. 

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