High-performance liquid chromatography pharmaceuticals

Rahavendran SV, Karnes HT. Solid-state diode laser-induced fluorescence detection in high-performance liquid-chromatography. Pharmaceutical Research 10, 328-334, 1993. 

Colin, H. and Krstulovic, A. M., The theory of retention in reversed-phase high-performance liquid chromatography, in Liquid Chromatography in Pharmaceutical Development, Wainer, I. W., Ed., Aster Publishing, 1985, 171. 

Capillary electrophoresis employing chiral selectors has been shown to be a useful analytical method to separate enantiomers. Conventionally, instrumental chiral separations have been achieved by gas chromatography and by high performance liquid chromatography.127 In recent years, there has been considerable activity in the separation and characterization of racemic pharmaceuticals by high performance capillary electrophoresis, with particular interest paid to using this technique in modem pharmaceutical analytical laboratories.128 130 The most frequently used chiral selectors in CE are cyclodextrins, crown ethers, chiral surfactants, bile acids, and protein-filled... 

R. Ricker, L. Sandoval, B. Permar, and B. Boyes, Improved reversed-phase high performance liquid chromatography columns for biopharmaceutical analysis, J. Pharmaceut. Biomed. Anal., 14, 93 (1995). 

The use of direct UV spectrophotometry to measure sample concentrations in pharmaceutical research is uncommon, presumably due to the prevalence and attractiveness of high-performance liquid chromatography (HPLC) and liquid chromatography/mass spectrometry (LC/MS) methods. Consequently, most researchers are unfamiliar with the value of UV detection, mainly that it is generally much faster than other methods - a very important asset in high-throughput screening. 

Alhaique et al. [62] used a reversed phase high performance liquid chromatography method for the determination of miconazole in bulk or pharmaceuticals using bezafibrate as internal standard. 

M. J. Dubber, V. Sewram, N. Mshicileli, G.S. Shephard and I. Kanfer, The simultaneous determination of selected flavonol glycosides and aglycones in Ginkgo biloba oral dosage forms by high performance liquid chromatography electrospray ionization mass spectrometry, J. Pharmaceut. Biomed., 37, 723 731 (2005). 

Separation-based techniques, especially high-performance liquid chromatography (HPLC) and gas chromatography (GC), have long been the work horses of pharmaceutical analysis laboratories. They are among the most powerful and versatile tools for the detection and quantitation of analytes (chemical components) in complex matrices frequently encountered in the course of PhR D. 

R.D. Voyksner, C.S. Smith and P.C. Knox, Optimization and application of particle beam high-performance liquid chromatography/mass spectrometry to compounds of pharmaceutical interest, Biomed. Environ. Mass Spectrom., 19 (1990) 523-534. 

J. O. Miners, The Analysis of Penicillins in Biological Fluids and Pharmaceutical Preparations by High-Performance Liquid Chromatography A Review , J. Liq. Chromatogr. 1995, 8, 2827-2843. 

Lou, D.-W., Saito, Y., and Jinno, K. (2005). Solid-phase extraction and high-performance liquid chromatography for simultaneous determination of important bioactive ginsenosides in pharmaceutical preparations. Chromatographia 62, 349-354. 

For the pharmaceutical scientist, understanding the theory and application of the equipment is usually not sufficient there is the matter of compliance. The qualification of CE is similar to that of other instruments. Installation qualification (IQ), operational qualification (OQ), and performance qualification (PQ) apply in much the same way as high-performance liquid chromatography (HPEC). This chapter details the different parts of the modern CE instrument, equipment-related issues and troubleshooting, instrument qualification, and the future of the CE instrument. 

Wynia, G. S., Windhorst, G., Post, P. C., and Maris, F. A. (1997). Development and validation of a capillary electrophoresis method within a pharmaceutical quality control environment and comparison with high-performance liquid chromatography. /. Chromatogr. A 773(1—2), 339—350. 

T.R.M. De Beer, W.R.G. Baeyens, A. Venneire, D. Broes, J.R Remon, and C. Vervaet, Raman spectroscopic method for the determination of medroxyprogesterone acetate in a pharmaceutical suspension Validation of quantifying abilities, uncertainty assessment and comparison with the high performance liquid chromatography reference method. Anal. Chim. Acta, 589, 192-199 (2007). 

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