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High-performance liquid chromatography oligosaccharides

Analyses were performed by gel permeation chromatography (GPC) and by high performance liquid chromatography (HPLC). Gel filtration of oligosaccharides was effected in a thermostated (65 <>C) column (210 X 1.5 i.d.) filled with polycrylamide gel (Bio-gel P2, 200-400 mesh Bio Rad-USA), using stilled water as eluent (flow rate 30 ml.h-1). [Pg.551]

Peelen, G. O. H., de Jong, J. G. N., and Wevers, R. A., High-performance liquid chromatography of monosaccharides and oligosaccharides in a complex biological matrix, Anal. Biochem., 198, 334, 1991. [Pg.281]

Suzuki-Sawada, J., Umeda, Y., Kondo, A., and Kato, I., Analysis of oligosaccharides by on-line high-performance liquid chromatography and ion-spray spectrometry, Anal. Biochem., 207, 203, 1992. [Pg.283]

Green, E. D. and Baenziger, J. U., Separation of anionic oligosaccharides by high-performance liquid chromatography, Anal. Biochem., 158, 42, 1986. [Pg.284]

Daniel PF, De Feudis DF, Lott IT, McCluer RH (1981) Quantitative microanalysis of oligosaccharides by high performance liquid chromatography. Carbohydr Res 97 161-180 De Jong JG, Aerts JM, van Weely S, et al (1998) Oligosaccharide excretion in adult Gaucher disease. J Inherit Metab Dis 21 49-59... [Pg.331]

Fig. 4.5.2 Actual strategies for CDG diagnosis. Initial investigations on CDG patients are routinely carried out by isoelectric focusing (IEF) of serum transferrin. With a CDG type I pattern, subsequent analysis should imply determination of phosphomannomutase (PMM) and phos-phomannose isomerase (PMI) activities. Further studies, like analysis of the lipid-linked- and protein-bound-oligosaccharides, determination of enzyme or sugar transporter activities and molecular biology studies often have to be performed in more specialised laboratories. HPLC High-performance liquid chromatography, TLC thin-layer chromatography... Fig. 4.5.2 Actual strategies for CDG diagnosis. Initial investigations on CDG patients are routinely carried out by isoelectric focusing (IEF) of serum transferrin. With a CDG type I pattern, subsequent analysis should imply determination of phosphomannomutase (PMM) and phos-phomannose isomerase (PMI) activities. Further studies, like analysis of the lipid-linked- and protein-bound-oligosaccharides, determination of enzyme or sugar transporter activities and molecular biology studies often have to be performed in more specialised laboratories. HPLC High-performance liquid chromatography, TLC thin-layer chromatography...
Table 4.5.6 Running conditions for high-performance liquid chromatography analysis of 2-aminobenzamide-labelled oligosaccharides... Table 4.5.6 Running conditions for high-performance liquid chromatography analysis of 2-aminobenzamide-labelled oligosaccharides...
M. Davies, K. D. Smith, A. M. Harbin, and E. F. Hounsell, High-performance liquid chromatography of oligosaccharide alditols and glycopeptides on a graphitized carbon column, J. Chromatogr., 609 (1992) 125-131. [Pg.132]

B. Barroso, R. Dijkstra, M. Geerts, F. Lagerwerf, P. van Veelen, and A. de Ru, On-line high-performance liquid chromatography/mass spectrometric characterization of native oligosaccharides from glycoproteins, Rapid Common. Mass Spectrom., 16 (2002) 1320-1329. [Pg.133]

B. Kiister, S. F. Wheeler, A. P. Hunter, R. A. Dwek, and D. J. Harvey, Sequencing of AMinked oligosaccharides directly from protein gels In-gel deglycosylation followed by matrix-assisted laser desorption/ionization mass spectrometry and normal-phase high-performance liquid chromatography, Anal. Biochem., 250 (1997) 82-101. [Pg.140]

Rudd PM, Colominas C, Royle L, Murphy N, Hart E, Merry AH, Hebestreit HF, Dwek RA. A high-performance liquid chromatography based strategy for rapid, sensitive sequencing of N-linked oligosaccharide modifications to proteins in sodium do-decyl sulphate polyacrylamide electrophoresis gel bands. Proteomics 2001 1 285-294. [Pg.750]

In our laboratory an authentic in vitro cellular model for Sandhoff disease has been generated upon treatment of RAW264.7 murine macrophages with an inhibitor of p-hexosaminidase (Boomkamp and Butters, unpublished data). High performance liquid chromatography (HPLC) analyses of extracted GSL oligosaccharides show that GA2 predominantly accumulates in inhibitor-treated RAW cells as opposed to a minor but significant increase in GM2 levels (Fig. 17.7). This difference in elevation is possibly due to the... [Pg.456]

Tomiya, N., et al. (1987). Structural Analysis of N-linked Oligosaccharides by a Combination of Glycopeptidase, Exoglycosidase and High-performance Liquid Chromatography, Anal. Biochem. 163 489-499. [Pg.220]

Mellis, S., and Banziger, J. (1981). Separation of Neutral Oligosaccharides by High-performance Liquid Chromatography, Ara/. Biochem. 114 276-280. [Pg.221]

Wang, W., et al. (1990). High-performance Liquid Chromatography of Sialic Acid-containing Oligosaccharides and Acidic Monosaccharides, . dna/. Biochem. 190 182—187. [Pg.221]

Aqueous and methanolic solutions of cyclodextrins have been employed as mobile phases in high performance liquid chromatography (HPLC), and as stationary phases by bonding the cyclodextrin to silica packing by several workers (1-8). They have been shown to be especially suitable for separation of structural isomers, cis-trans geometric isomers, and enantiomers. Cyclodextrins (CD) are toroidal-shaped, cyclic, oligosaccharides made up of o-l,4 linked,... [Pg.226]

Honda, S. High-performance liquid chromatography of mono- and oligosaccharides. Anal Biochem, 140, 1,1984. [Pg.285]

Kamoda, S., Nakano, M., Ishikawa, R., Suzuki, S. and Kakehi, K. Rapid and sensitive screening of N-glycans as 9-fluorenylmethyl derivatives by high-performance liquid chromatography a method which can recover free oligosaccharides after analysis. J Proteome Res, 4, 146, 2005. [Pg.290]

P.J. Simms, R.M. Haines and K.B. Hicks, High-Performance Liquid Chromatography of Neutral Oligosaccharides on a P-Cyclodextrin Bonded Phase Column, J. Chromatogr., 648(1993)131. [Pg.492]

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See also in sourсe #XX -- [ Pg.479 ]



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