High-performance liquid chromatography methodology

W. Mulholland, P. L. Naish, D. R. Stout, and J. Waterhouse, Experimental design for the ruggedness testing of high performance liquid chromatography methodology, Chemom. Intell. Lab. Syst., 5, 263-270 (1989). 

Dias R.C.E., Campanha F.G., Vieira L.G.E., Ferreira L.P., Pot D., Marraccini P., De Toledo Benassi M. Evaluation of kahweol and cafestol in coffee tissues and roasted coffee hy a new high-performance liquid chromatography methodology. Journal of Agricultural and Food Chemistry, 58 88-93 (2010). 

Parallel to the development of mass spectrometric instrumentation and methodologies, the improvements of separation techniques, such as gas chromatography (GC), high performance liquid chromatography (HPLC) and capillary electrophoresis (CE), and of their coupling with MS allowed the study of complex mixtures, that are generally encountered in most studies. 

Note. Abbreviations g.l.c., gas-liquid chromatography e.i.-m.s., electron-impact mass spectrometry c.i.-m.s., chemical-ionization mass spectrometry h.p.l.c., high-performance liquid chromatography h.p.a.e., high pH anion-exchange f.a.b.-m.s., fast-atom-bombardment mass spectrometry l.s.i.-m.s., liquid secondary-ion mass spectrometry n.O.e., nuclear Overhauser enhancement. Details of these methodologies are given in Ref. (3). 

Several techniques have been developed for the trace analysis of cationic surfactants. Most of the methodologies are based on high-performance liquid chromatography (HPLC) techniques, because most of the commercial cationic surfactants are produced as homologous... 

SONG w o, BEECHER G R and eitenmiller R R (2000), Modern Analytical Methodologies In Fat- and Water-soluble Vitamins. Chichester, Wiley. sprenger c, galensa r and jensen d (1999), Simultaneous determination of cellobiose, maltose and maltotriose in fruit juices by high-performance liquid chromatography with biosensor detection , Dtsch Lebensm Rundsch, 95, 499-504. 

Nowadays, the preferred methodology is the separation and quantification of porphyrins by high-performance liquid chromatography (HPLC) with fluorescence detection. Thus, a method that detects the main isomers of specific porphyrins is recommended, as a high number of different isomers exist, especially in pathological conditions, and the relative abundance of the different isomers supports the distinction of the different porphyrias (Table 7.3.1). 

E. Ramalhosa, S. Rio Segade, E. Pereira, C. Vale and A. Duarte, Simple methodology for methylmercury and inorganic mercury determinations by high-performance liquid chromatography-cold vapour atomic fluorescence spectrometry. Anal. Chim. Acta, 448(1-2), 2001, 135-143. 

MV Bell. Separations of molecular species of phospholipids by high performance liquid chromatography. In WW Christie, ed. Advances in lipid methodology—four. Dundee, Scotland Oily Press, 1997, pp 45-82. 

B Zimmerli, R Dick. Determination of ochratoxin A at the ppt level in human blood, serum, milk and some foodstuffs by high-performance liquid chromatography with enhanced fluorescence detection and immunoaffinity column cleanup methodology and Swiss data. J Chrom B 666 85-99,1995. 

In most common turbulent-flow chromatography experiments, a second conventional high-performance liquid chromatography (HPLC) column is employed. The conventional HPLC column, usually referred to as the analytical column, is used for peak sharpening and additional separation prior to MS detection. This approach requires an additional valve and pump to control the final HPLC separation. A typical dual-column methodology is shown in Fig. 10.5. 

Padron Sanz, C., Z. Sosa Ferrera, and J.J. Santana Rodriguez. 2002. Extraction and preconcentration of polychlorinated dibenzo-p-dioxins using the cloud-point methodology. Application to their determination in water samples by high-performance liquid chromatography. Anal. Chim. Acta 470 205-214. 

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