High-performance liquid chromatography carbohydrates

Daniel PF, De Feudis DF, Lott IT, McCluer RH (1981) Quantitative microanalysis of oligosaccharides by high performance liquid chromatography. Carbohydr Res 97 161-180 De Jong JG, Aerts JM, van Weely S, et al (1998) Oligosaccharide excretion in adult Gaucher disease. J Inherit Metab Dis 21 49-59... 

Yamauchi, S., Nimura, N., and Kinoshita, T., Protamine-coated silica gel as packing material for high performance liquid chromatography of carbohydrates, Analyst, 118, 161, 1993. 

FEURLE, J., JOMAA, H., WILHELM, M GUTSCHE, W.B., HERDRICH, M., Analysis of phosphorylated carbohydrates by high-performance liquid chromatography-electrospray ionization tandem mass spectrometry utilising p-cyclodextrin bonded stationary phase, J. Chromatogr., 1998,803,111-119. 

High-performance liquid chromatography of carbohydrates, 46, 17-72 addendum to, 46, 327 - 329... 

Despite the advances made in high-performance liquid chromatography in recent years, there are still occasionally applications in which conventional column chromatography is employed. These methods lack the sensitivity, resolution and automation of HPLC. They include the determination of urea herbicides in soil, polyaromatic hydrocarbons, carbohydrates, chloroaliphatic compounds and humic and fulvic acids in non-saline sediments. The technique has also been applied in sludge analysis, e.g. aliphatic hydrocarbons and carboxylic acids. 

Townsend, R. R. (1995). Carbohydrate Analysis High-Performance Liquid Chromatography and Capillary Electrophoresis, Elsevier, New York. 

Wei Y, Ding MY. Analysis of carbohydrates in drinks by high-performance liquid chromatography with a dynamically modified amino column and evaporative light scattering detection. Journal of Chromatography A 904, 113-117, 2000. 

However, during the past three decades, an analytical method has been developed that currently rivals and may soon surpass the traditional liquid chromatographic techniques in importance for analytical separations. This technique, high-performance liquid chromatography (HPLC), is ideally suited for the separation and identification of amino acids, carbohydrates, lipids, nucleic acids, proteins, pigments, steroids, pharmaceuticals, and many other biologically active molecules. 

Honda, S., Kakehi, K., Fujikawa, K., Oka, Y., and Takahashi, M. 1988. Mechanism of the reaction of reducing carbohydrates with 2-cyanoacetamide, used for postcolumn labeling in high performance liquid chromatography for photometric, fluorimetric and electrochemical detection. Carbohydr. Res. 183 59-69. 

The analysis of food carbohydrates by high-performance liquid chromatography (HPLC) has been dealt with and reviewed by several authors, either as books or as chapters of books or as scientific papers, most notably in the current decade (1-6). 

High-performance liquid chromatography remains the most widely used technique in the determination of sugars in foodstuffs. In fact, all those foods in which free carbohydrates occur significantly have been analyzed by means of this technique. 

Fig. 4.3. High performance liquid chromatography (HPLC) of the monosaccharides obtained from a partially purified preparation of microbubble glycopeptide surfactant from forest soil. Following hydrolysis (in 2 N HC1 for 6 hr at 100°C) and filtration, the carbohydrate mixture was charged on a Bio-Rad HPX-87 cation exchange column. For comparison, part A shows the chromatogram (using the same HPLC column) of a standard solution, which contained 4 pg of each of three different monosaccharides (i.e., the last three peaks shown are glucose, xylose and fiicose, in the order of increasing retention times). Part B shows the chromatogram obtained from hydrolysis of the partially purified (see text) microbubble surfactant (approximately 30 pg). All other experimental conditions were identical in the two cases, i.e., water eluent, 0.5 ml/min flow rate, 85°C, refractive index detector attenuation -2x. (Taken from ref. 322.)...

M. McNeil, A. G. Darvill, P. Aman, L. E. Franzen, and P. Albersheim, Structural analysis of complex carbohydrates using high performance liquid chromatography, gas chromatrography and mass spectrometry, Meth. Enzymol., 83 (1982) 3 15. 

R. W. Kane and J. D. Timpa, A high-performance liquid chromatography study of D-cellobiose degradation under Fenton conditions, J. Carbohydr. Chem., 11 (1992) 779-797. 

Lehrfeld, J., Separation of some perbenzoylated carbohydrates by high performance liquid chromatography, 7. Chromatogr., 120, 141, 1976. 

Two aliquots of each sample were analyzed by quantitative saccharification for each of the three replicate columns at each condition, for a total of 12 independent measurements of each composition. Carbohydrate analyses were done by high-performance liquid chromatography using a Bio-Rad HPX-87P carbohydrate column as previously described... 

Ruiz, R. and Ehrman, T. (1996), Determination of Carbohydrates in Biomass by High Performance Liquid Chromatography, Laboratory Analytical Procedure No. LAP-002, National Renewable Energy Laboratory, Golden, CO. 

U. Karsten, D. N. Thomas, G. Weykam, C. Daniel, and G. O. Kirst, A simple and rapid method for extraction and separation of low molecular weight carbohydrates using high-performance liquid chromatography, Plant Physiol. Biochem., 29 (1991) 373-378. 

High Performance Liquid Chromatography. Tissue extracts were analyzed with a Varian model 5020 liquid chromatograph equipped with a Rheo-dyne model 7120 loop injector valve, a Tracer 970 variable wavelength detector set at 257 nm, an automated Hewlett-Packard 3385A printer-plotter system for determining retention times and peak areas, and a Waters /x Bondapak column (3.9 mm i.d. X 300 mm) for carbohydrate analysis. The buffer was eluted isocratically at 1 mL/min with a 1 4 (v/v) mixture of 0.01 M monobasic sodium phosphate (pH 4.46) and methanol. The minimum amount detectable was 10 ng. 

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