High performance liquid chromatography capillary zone electrophoresis

Figure 9.6 Surfer-generated chromatoeletropherogram of fluorescamine-labeled tryptic digest of ovalbumin. Reprinted from Analytical Chemistry, 62, M. M. Bushey and J. W. Jorgenson, Automated instrumentation for comprehensive two-dimensional high-performance liquid chromatography/capillary zone electrophoresis, pp 978-984, copyright 1990, with permission from the American Chemical Society.

PACKED CAPILLARY REVERSE PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY-CAPILLARY ZONE ELECTROPHORESIS... 

Rathore, A. S., and Horvath, C. S. (1996). Separation parameters via virtual migration distances in high-performance liquid chromatography, capillary zone electrophoresis and electrokinetic chromatography /. Chromatogr. A 743, 231-246. 

Cloud point extraction has been applied to the separation and preconcentration of analytes including metal ions, pesticides, fungicides, and proteins from different matrices prior to the determination of the analyte by techniques such as atomic absorption, gas chromatography, high performance liquid chromatography, capillary zone electrophoresis, etc. 

Figure 2.1 Mass spectrometric approach. Dl, direct inlet GC, gas chromatography HPLC, high performance liquid chromatography CZE, capillary zone electrophoresis El, electron ionization Cl, chemical ionization ESI, electrospray ionization DESI, desorption electrospray ionization APCI, atmospheric pressure chemical ionization MALDI, matrix assisted laser desorption ionization B, magnetic analyzer E, electrostatic analyzer...

C Miller, J Rivier. Peptide chemistry development of high performance liquid chromatography and capillary zone electrophoresis. Biopolymers (Pept Sci) 40, 265, 1996. 

Reilly, J., and Saeed, M. (1998). Capillary electrochromatography as an alternative separation technique to high-performance liquid chromatography and capillary zone electrophoresis for the determination of drug related impurities in Lilly compound LY300164. /. Chromatogr. A 829, 175-186. 

CZE = capillary zone electrophoresis EC = electrochemical detector GC = gas chromatography HCD = Hall conductivity detector HPLC = high performance liquid chromatography IDMS = isotope dilution mass spectrometry MS = mass spectrometry RSD = relative standard deviation SEE = supercritical fluid extraction SPE = solid phase extraction UV = ultraviolet absorbance detection... 

For the separation of C-glycosylflavones, high-performance liquid chromatography (HPLC) has been mainly used in recent works. However, we have to underline a few interesting contributions on the performance of capillary zone electrophoresis (CZE) in this field. 

Triethanolamine can be determined in metalworking and cutting fluids by gas chromatography-mass selective detection of silylated derivatives, by isotachophoresis, by capillary zone electrophoresis with indirect ultraviolet detection, and by spectrophotometry (Kenyon et al, 1993 Fernando, 1995 Schubert et al, 1996 Sollenberg, 1997) and in cosmetics and pharmaceuticals by ion-exclusion chromatography and by reversed-phase high performance liquid chromatography (Fukui et al, 1992 Maurer etal, 1996). 

M Jimidar, TP Hamoir, A Foriers, DL Massart. Comparison of capillary zone electrophoresis with high-performance liquid chromatography for the determination of additives in foodstuffs. J Chromatogr 636 179-186, 1993. 

Steuer et al. compared supercritical fluid chromatography with capillary zone electrophoresis (CZE) and high-performance liquid chromatography (HPLC) for its application in pharmaceutical analysis [24]. Efficiency, performance, sensitivity, optimization, sample preparation, ease of method development, technical capabilities, and orthogonality of the information were the parameters studied. They concluded that SFC is ideal for moderately polar compounds, such as excipients, for which mass detection is required. 

W. Steuer, I. Grant, and F. Erni, Comparison of high-performance liquid chromatography, Supercritical fluid chromatography and capillary zone electrophoresis in drug analysis, J. Chromatogr., 507 125 (1990). 

Rudnick, S. E., Hilser, V. J., Jr., and Worosila, G. D. (1994). Comparison of the utility of capillary zone electrophoresis and high-performance liquid chromatography in peptide mapping and separation. J. Chromatogr. 672, 219-229. 

Capillary zone electrophoresis is also complementary to high-performance liquid chromatography (HPLC), as is shown by the application of these methods in purity assessment of peptides (see Fig. 2).lla... 

Garcia-Viguera, C., Bridle, P. (1995). Analysis of non-coloured phenolic compounds in red wines. A comparison of High-Performance Liquid Chromatography and Capillary Zone Electrophoresis. Food Chem., 54, 349-352. 

Terzuoli, L. Porcelh, B. Setacci, C. Giubbolini, M. Cinci, G. Carlucci, F. Pagani, R. MarineUo, E. Comparative determination of purine compounds in carotid plaque by capillary zone electrophoresis and high-performance liquid chromatography. J. Chromatogr., B 1999, 728, 185-192. 

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