High performance liquid chromatography buffers used

Finally, levels of furfural and 5-hydroxymethyl-2-furfuraldehyde have also been monitored in processed fruit juices by a high performance liquid chromatography method using a reversed-phase macroreticular column and phosphate buffer as eluent [399], These aldehydes are degradation products of L-ascorbic acid. The detection limit of this method was reported as 0.05 mg r which was insufficiently sensitive to detect the presence of furfural. 

In the separation of biomolecules, sample preparation almost always involves the use of one or more pretreatment techniques. With high-performance liquid chromatography (HPLC), no one sample preparation technique can be appHed to all biological samples. Several techiques may be used to prepare the sample for injection. For example, complex samples require some form of preffactionation before analysis, samples that are too dilute for detection require concentration before analysis, samples in an inappropriate or incompatible solvent require buffer exchange before analysis, and samples that contain particulates require filtration before injection into the analytical instrument. 

Determination of brinzolamide and its 3 principal metabolites (the N-desethyl, A -desmethoxypropyl and O-desmethyl analogs) in whole blood and plasma from clinical and pre-clinical studies was performed using high performance liquid chromatography (HPLC) with UV detection. After addition of a known amount of internal standard (AL-5138, the 4-methoxybutyl analog of brinzolamide), the sample was acidified with 50 mM sodium phosphate buffer, pH 3.0 and extracted with ethyl acetate. 

Evaporate a 5- ll aliquot to dryness under a vacuum. Redissolve the residue in a suitable buffer prior to analysis by an amino acid analyzer or into the appropriate eluant if high performance liquid chromatography (HPLC) analysis is used. 

Development of fast, accurate, and reproducible high-performance liquid chromatography (HPLC) methods has offset the use of traditional open-column and TLC methods in modern chlorophyll separation and analysis. A number of normal and reversed-phase methods have been developed for analysis of chlorophyll derivatives in food samples (unit F4.4), with octadecyl-bonded stationary phase (C]8) techniques predominating in the literature (Schwartz and Lorenzo, 1990). Inclusion of buffer salts such as ammonium acetate in the mobile phase is often useful, as this provides a proton equilibrium suitable for ionizable chlorophyllides and pheophorbides (Almela et al., 2000). 

For small molecule analytes (see Note 6) for which a radiotracer form is available, sequentially load a known quantity of tracer dissolved in buffer and determine the amount of analyte in the eluant. When the radioactivity not retained by the immunoaffinity column plateaus, the column binding sites are saturated. Wash the column, and elute the retained radioactivity. The mass of analyte in the eluted volume is the apparent column capacity. In many instances a radio-labeled analyte may not be available. In such cases, high-performance liquid chromatography, UV spectroscopy, or any other analytical tool capable of selectively quantifying the analyte may be used to determine column capacity. 

The use of high performance liquid chromatography (HPLC) on-line or off-line is an essential feature for peptide mapping to integrate the removal of buffers and salts (purification) and the separation of analytes (preconcentration) with mass spectrometry. With on-line LC/MS approaches, low flow rates (<100pL/min) have been demonstrated to provide maximum sensitivity with ESI techniques for the analysis of proteins. In the work performed by Arnott and... 

Prior to high-performance liquid chromatography (HPFC), samples were filtered through a 0.2-gm pore size mixed cellulose ester filter (Schleicher Schuell, Dassel, Germany). Buffer components such as acetic acid, citric acid, maleic acid, and succinic acid were separated on an Aminex HPX-87H (Bio-Rad, Hercules, CA) organic acid column at 65°C using a... 

High-performance liquid chromatography (HPLC) is a well-established separation technique it is able to solve numerous analytical problems and there is the possibility of acting on the mobile phases with appropriate additives to improve the quality of the peak. Of course, any additive must be compatible with the MS detector nonvolatile buffer or eluent additives cannot be used strong acids such as trifhioroacetic acid (TFA) may cause significant signal suppression in positive ionization. Different stationary phases are used as an alternative to the classical C18 Phenyl, HILIC, fluorinated, etc. 

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