High performance liquid chromatography ascorbate determination

Feubolt, R. and Klein, H., Determination of sulphite and ascorbic acid by high-performance liquid chromatography withe electrochemical detection,. Chromatogr., 640, 271, 1993. 

H Iwase, I Ono. Determination of ascorbic acid and dehydroascorbic acid in juices by high-performance liquid chromatography with electrochemical detection using L-cysteine as precolumn reduc-tant. J Chromatogr 654 215-220, 1993. 

J Carnevale. Determination of ascorbic, sorbic and benzoic acids in citrus juices by high-performance liquid chromatography. Food Tech Aust 32 302, 304-305, 1980. 

Willson et al. (13) note that most analytical techniques (e.g., high-performance liquid chromatography [HPLC]) look specifically for the arrival of a single product of the reaction (or loss of a reactant), and they are therefore not necessarily of value in investigations of intermediates in the reaction process, but that isothermal microcalorimetry follows the entire process (see earlier text). By comparison of calorimetric data and analytical data, it was possible to propose a sequential reaction mechanism for ascorbic acid oxidation that could not be determined simply from analytical data. 

Chemical additives used in baking and not already present in flour in the required quantities are added to the dough in the form of a premix. Osborne [17] used NIR in a model experiment to determine three bread improvers (ascorbic acid, L-cysteine, and azodicarbonamide) in admixture in a starch diluent. Although the accuracy of each calibration was not satisfactory for their determination at the levels encountered in bakery premixes, the results were sufficient to indicate the potential of NIR for this type of application. This potential has been realized in the determination of nicotinamide in nutrient premixes where Osborne [18] obtained excellent results by NIR compared with high-performance liquid chromatography. The NIR calibration involved a ratio of second derivatives at 2138 nm, corresponding to a nicotinamide absorption band, and 2070 nm as a reference. 

Iwase, H. (2000) Use of nucleic acids in the mobile phase for the determination of ascorbic acid in foods by high-performance liquid chromatography with electrochemical detection. J. Chromatogr. A, 881, 327-330. 

T Ito, H Murata, Y Yasui, M Matsui, T Sakai, K Yamauchi. Simultaneous determination of ascorbic acid and dehydroascorbic acid in fish tissues by high-performance liquid chromatography. J Chromatogr 667 355-357, 1995. 

G Barja de Quiroga, M Ldpez-Torres, R Perez-Campo, C Rojas. Simultaneous determination of two antioxidants, uric and ascorbic acid, in animal tissue by high-performance liquid chromatography. Anal Biochem 199 81-85, 1991. 

LS Liau, BL Lee, AL New, CN Ong. Determination of plasma ascorbic acid by high-performance liquid chromatography with ultraviolet and electrochemical detection. J Chromatogr 612 63-70, 1993. 

Ziegler SJ, Meier B, Sticher O. Rapid and sensitive determination of dehydroascorbic acid in addition to ascorbic acid by reversed-phase high performance liquid chromatography using a post-column reduction system. J Chromatogr A 1987 391 419-26. 

FIGURE 18.3 Distribution of flow injection analysis (FIA) determinations of ascorbic acid (AA) according to the detection technique used. C calorimetry CE capillary electrophoresis HPLC high-performance liquid chromatography MS mass spectrometry. 

Kutnink, M. A. and S. T. Omaye. 1987. Determination of ascorbic acid, erythorbic acid, and uric acid in cured meats by high performance liquid chromatography. J. Food Sci. 52 53-56. 

Gazdik, Z., O. Zitka, J. Petrlova et al. 2008. Determination of vitamin C (ascorbic acid) using high performance liquid chromatography coupled with electrochemical detection. Sensors 8 7097-7112. 

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