High-performance liquid chromatography and advanced separation technologies

4 High-performance liquid chromatography and advanced separation technologies 

High-performance liquid chromatography (HPLC) has emerged as one of the most useful tools for the bioanalytical laboratory. This is a powerful stand-alone method that can be used for the purification, separation and quantification of biomolecules. Notably HPLC analysis can be combined with other high-tech methods such as mass spectrometry (MS) or nuclear magnetic resonance (NMR) spectroscopy to enable the identification of biomolecules. 

Advantages of HPLC over classical chromatography methods 

HPLC is a very versatile method encompassing all major forms of liquid chromatography. In essence, HPLC is an advanced automated from of partition, adsorption, ion-exchange, size-exclusion, and more recently affinity chromatography. 

ENDCAPPING Remaining -OH groups on the silica are blocked with Trimethylchlorosllahe to prevent these polar groups trom interacting with solute, 

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