High performance chelation ion chromatography

Pauli B, Foulkes M, Jones P. 1994. Determination of alkaline earth metals in offshore oil-well brines using high-performance chelation ion chromatography. Anal Proc 31 209-211. 

Truscott, J. B., Jones, P., Fairman, B. E., and Evans, E. H., Determination of actinides in environmental and biological samples using high-performance chelation ion chromatography coupled to sector-field inductively coupled plasma mass spectrometry, J. 

Figure 2 Isocratic separation of standard mixture of 14 lanthanides and yttrium on a 250 mm x 4 mm column, packed with 5 pm silica IDA. Eluent 1.6x 10" moll" HNO3 with 0.5moll KNO3 flow rate 1.0 ml min " k column temperature 65°C, sample volume 20 pi, sample concentration of each metal was 4 ppm in 0.2% HNO3. Detection, Arsenazo III postcolumn reaction at 658 nm. (Reprinted with permission from Nesterenko PN and Jones P (1998) Isocratic separation of lanthanides and yttrium by high performance chelation ion chromatography. Journal of Chromatography A 804 223-231 Elsevier.)...

Figure 3 The separation of Ba(ll) 1.5 ppm, Ni(ll) 1 ppm, Co(ll) 2 ppm, Zn(ll) 1 ppm, Pb(ll) 5 ppm, Cd ll) 2.5 ppm, Mn(ll) 1.5 ppm, and Cu(ll) 5 ppm on the aminophosphonic acid functionalized silica column, 250 mm x 4.6 mm. Eluent ImolT KNO3, 5 mmol 1 HNO3. Detection, PAR/ZnEDTA postcolumn reaction at 495 nm. (Reprinted with permission from Nesterenko PN, Shaw MJ, Hill S, and Jones P (1999) Aminophosphonate-functionalised silica A versatile chromatographic stationary phase for high performance chelation ion chromatography. Microchemical Journal 62-. 58-69 Elsevier.)...

Figure 8 Chromatogram showing the separation of barium and strontium from magnesium and calcium in an oil-well brine (sample 1, diluted 1 1 v/v with double distilled water) using a Methyl-thymol Blue impregnated column (pH 9.2). (Determination of alkaline earth metals in offshore oil-well brines using high performance chelation ion chromatography. Pauli B, Foulkes M, and Jones P (1994) Analytical Proceedings 31 209-211 reproduced by permission of The Royal Society of Chemistry.)...

Jones P and Nesterenko PN (1997) High performance chelation ion chromatography a new dimension in the separation and determination of trace metals. Journal of Chromatography 789 413—435. 

Nesterenko P.N. Jones, P. Recent developments in the high-performance chelation ion chromatography of trace metals. J. Sep. Sci. 2007, 30,1773-1793. 

P. N. Nesterenk and P. Jones, Isocratic separation of lanthanides and yttrium by high performance chelation ion chromatography on iminodiacetic acid bonded to silica,/. Chromatogr. A, 804, 223,1998. 

Nesterenko PN, Jones P, Pauli B. High performance chelation ion chromatography. Cambridge, England Royal Society of Chemistry 2011. 

High-performance chelation ion chromatography involving dye-coated resins Spec determination of the colored complex in a phosphoric acid medium UV spec A = 320 nm... 

The selectivity characteristics of high performance chelation ion chromatography when separating a range of metal ions with a number of complexing eluents has been investigated (71). 

Chung, Y. and Chung, W., Determination of Co(II) ion as a 4-(2-thiazolylazo)resorcinol or 5-methyl-4-(2-thiazolylazo)resorcinol chelate by reversed-phase capillary high-performance liquid chromatography. Bulletin of the Korean Chemical Society 24(12), 1781-1784, 2003. 

Purification performance of metal chelate affinity chromatography for antibodies can be very attractive as very high purity levels in a single pass have been reported.191 Known contaminants of purified antibodies using metal chelate affinity chromatography include transferrin, and albumin traces. Eluted antibodies can also be contaminated by significant amounts of metal ions that must be removed for the following separation or before the final formulation is performed. 

Srijaranai, S. et al. Flow-injection in-line complexation for ion-pair reversed phase high performance liquid chromatography of some metal -(2-pyridylazo) resorcinol chelates. Talanta 2006, 68, 1720-1725. 

Zacharion, M. Traverso, L Hearn, M.T.W. High-performance liquid chromatography of amino acids, peptides and proteins CXXXI. o-Phosphoserine as a new chelating ligand for use with hard Lewis metal ions in the im-mobilized-metal affinity chromatography of proteins. J. Chromatogr. 1993, 646, 107-120. 

Rigidity and mechanical stability of the substrate under conditions of high-performance liquid chromatography are important and microspherical particles of macroporous highly crosslinked PS-DVB or poly(metacrylate) and silica are well suited for CIC. There are three main methods of producing a chelating ion-exchange surface. 

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