Hexose oxidase

The main problem of the function of oxidases is their need for a substrate as energy source. This problem was successfully addressed by Kristensen et al., who created a coating that contained starch, glucoamylase, and hexose oxidase... 

Hexose oxidase Chondrus crispus Increase gluten strength... 

Poulsen, C., and P. B. Hostrup. 1998. Purification and Characterization of a Hexose Oxidase with Excellent Strengthening Effeets in Bread. Cereal Chemistry Journal 75 (l) 51-57. 

False. D-gluconolactone is produced directly from glucose via glucose oxidase. 6-phosphogluconolactone is an intermediate in the hexose monophosphate pathway. 

Superoxide is produced by the NADPH oxidoreduc-tase (oxidase), which is a membrane-bound enzyme complex containing a flavoprotein that catalyses the transfer of single electrons from NADPH in the cytosol to extracellular oxygen. NADPH is mainly supplied by the hexose monophosphate shunt. In resting cells, the oxidase complex is inactive and disassembled, but is rapidly reconstituted and activated by chemotactic mechanisms or phagocytosis (Baggiolini and Thelen, 1991). 

The recent work of Blumenfeld ei al. (1963b), using periodate, indicated that in ichthyocol the hexoses are linked through the C-1 position while the 2-, 3-, and 4-hydroxyl groups are unsubstituted (see Section VI). To study the C-6 position of the galactose in ichthyocol they used galactose oxidase which will only oxidize galactose residues with the C-6 position unsubstituted. After treatment of ichthyocol with this enzyme 82 % of... 

A 30 cm Protein Pak 300 SW column (Waters product no. 80013) and isocratic Waters HPLC were used to perform the chromatography studies. Solvent flow rate was fixed at 1.0 ml/min and 10 ml samples of 1-10 mg/ml were injected. Eluted components were detected by uv absorption at 280 nm. The retention times of myoglobin (18,800 MW), a-chymotrypsin (25,000 MW), hexose kinase (100,000 MW), and glucose oxidase (186,000 MW) were determined in order to calibrate the column. 

At equilibrium, the ratio of maltose to D-glucose is 0.52. By use of substrates labeled with carbon-14, it was diown that the reducing group of the maltose molecule is liberated as free D-glucose, and the nonreducing residue is transferred to the donor substrate. In the presence of D-glucose oxidase, equilibrium is not established, and polysaccharide which is stained blue by iodine is synthesized. Amylomaltase thus superficially resembles D-enzyme, although it must be emphasized that maltose is not a substrate for D-enzyme, which transfers two or more hexose residues at a time. 

In between the lines of haptoglobin and a2-macroglobulin, the light line caused by ceruloplasmin is just visible (U2). It is the principal carrier of copper, 8 atoms per molecule besides, it contains 9.5 % of hexoses and has a molecular weight of 150,000 (S9). Its polyphenol-oxidase activity can be used for localization and, coupled with ImEl, for identification. 

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