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Heteronuclear NMR

ColEl Regulation by RNA Hairpins. Rephcation of the E. coli plasmid ColEl is regulated by two short RNA molecules and a protein in a system that provides an example of the unique stmcmral elements accessible to RNA molecules. Multidimensional heteronuclear nmr spectroscopy has been used to characterize the complex formed between the two RNAs (25). Each of the RNA molecules fold back on the other to form a pair of hairpin... [Pg.256]

Multidimensional and heteronuclear NMR techniques have revolutionised the use of NMR spectroscopy for the structure determination of organic molecules from small to complex. Multidimensional NMR also allows observation of forbidden multiple-quantum transitions and probing of slow dynamic processes, such as chemical exchange, cross-relaxation, transient Over-hauser effects, and spin-diffusion in solids. [Pg.338]

Three-Dimensional Structure of the Inhibited Catalytic Domain of Human Stromelysin-1 by Heteronuclear NMR Spectroscopy... [Pg.69]

Gooley PR, Johnson BA, Marcy AI, Cuca GC, Salowe SP, Hagmann WK, Esser CK, Springer JP. Secondary structure and zinc ligation of human recombinant short-form stromelysin by multidimensional heteronuclear NMR. Biochemistry 1993 32 13098-13108. [Pg.90]

Adamantoid systems [(//-ChR)6(MX)4]2 (Ch = S, Se, Te R usually Ph M = Cd, Hg X = halogene) have been prepared and studied by X-ray structure analyses, heteronuclear NMR and vibrational spectrometries, and partly by UV/vis spectrometry.337-341 Meanwhile, the systems have been expanded to superadamantoid clusters with four fused adamantoid subunits [MioCh ChPh fTPhAd342 343 and related clusters,344 and finally to nanoclusters with up to 32 metal atoms in one cluster.34, 3 6... [Pg.1283]

Pervushin KV, Orekhov V, Popov AI, Musina L, Arseniev AS (1994) Three-dimensional structure of (l-71)bacterioopsin solubilized in methanol/chloroform and SDS micelles determined by 15N-1H heteronuclear NMR spectroscopy. Eur J Biochem 219 571-583... [Pg.115]

CCR is easily measured by heteronuclear NMR experiments of isotopically labeled molecules. The information extracted from these experiments will significantly improve the resolution of NMR structures, especially bound conformations of weakly bound ligands, since /-couplings cannot be used in this case. The reason is the fact that the nonbound conformation significantly contributes to the averaged values of the coupling constant. [Pg.362]

Miuea, T., Kiaus, W., Gsell, B., Miyamoto, C. and Senn, H. Characterization of the binding interface between ubiquitin and class I human ubiquitin-conjugating enzyme 2b by multidimensional heteronuclear NMR spectroscopy in solution. J Mol Biol 1999, 290, 213-28. [Pg.189]

The solution structure of a thioredoxin from B. acidocaldarius (Topt = 60 °C) has been studied by NMR and compared with that of E. coli determined by X-ray analysis. It was found that the higher thermostability of the former is due to cumulative effects, the main factor being an increased number of ionic interactions cross-linking different secondary structural elements. Multidimensional heteronuclear NMR spectroscopy was also employed to characterize thioredoxin homologues found in the hyperthermophilic... [Pg.133]

The solution structures of 1 1 complex between paromomycin and gentamicin Cla and the A-site oligonucleotide have been solved at high resolution using heteronuclear NMR techniques These approaches require the preparation of uniformly labeled N- and C-RNA oligonucleotides via in vitro transcription with labeled nucleoside triphosphates. The use of RNAs labeled with NMR-active nuclei allows for the application of sophisticated heteronuclear NMR methods. These methods facilitate the assigmnent of NMR resonances and the acquisition of structural restraints for detailed structure determinations. [Pg.189]

Wang, Y.-S. Beyer, B. M. Senior, M. M. Wyss, D. F. Characterization of Autocatalytic Conversion of Precursor BACEf by Heteronuclear NMR Spectroscopy. Biochemistry 2003, 44, 16594-16601. [Pg.682]

Over the past decades several techniques have been used to obtain direct experimental information on the speciation of peroxo metal complexes heteronuclear NMR, IR and Raman spectroscopy, potentiometry and electrospray ionization mass spectrometry (ESI-MS). Often, more than one technique was used at the same time for acquiring complementary evidence. A significant, although nonexhaustive, list of examples related to several metals is given in Table 2. They include cases with hydrogen peroxide or alkyl... [Pg.1069]

NMR experiments. Heteronuclear NMR experiments are represented by H C HSQC (Figure 2.1) and heteronuclear multiple bond correlation HMBC) (Figure 2.2)... [Pg.43]


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See also in sourсe #XX -- [ Pg.318 , Pg.322 ]




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Heteronuclear 2D NMR

Heteronuclear NMR Spectroscopy

Heteronuclear NMR experiments

Heteronuclear NMR spectra

Multidimensional heteronuclear NMR

Multidimensional heteronuclear NMR experiment

Two-dimensional NMR heteronuclear multiple

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